Standard Curve (Sample)
(Fig.1. Human Neuronal-specific septin-3 (SEPT3) Standard Curve.)
Human Neuronal-Specific Septin-3 (SEPT3) ELISA Kit | SEPT3 elisa kit
Human Neuronal-Specific Septin-3 (SEPT3) ELISA Kit
Gene Names
SEPTIN3; SEPT3
Reactivity
Human
Synonyms
Neuronal-Specific Septin-3 (SEPT3); N/A; Human Neuronal-Specific Septin-3 (SEPT3) ELISA Kit; CTA-250D10.3; MGC133218; SEP3; bK250D10.3; neuronal-specific septin 3; SEPT3 elisa kit
Reactivity
Human
Specificity
Human Neuronal-specific septin-3 (SEPT3) ELISA Kit has high sensitivity and excellent specificity for detection of Human SEPT3. No significant cross-reactivity or interference between Human SEPT3 and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Neuronal-specific septin-3 (SEPT3) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Neuronal-specific septin-3 (SEPT3) Standard Curve.)
Related Product Information for SEPT3 elisa kit
Description: This Human Neuronal-specific septin-3 (SEPT3) ELISA Kit employs a two-site sandwich ELISA to quantitate SEPT3 in samples. An antibody specific for SEPT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SEPT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEPT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEPT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Neuronal-specific septin-3 belongs to the septin family of GTPases. Members of this family are required for cytokinesis. Expression is upregulated by retinoic acid in a human teratocarcinoma cell line. The specific function of this gene has not been determined. Alternative splicing of this gene results in multiple transcript variants. SEP3 shares almost 100% sequence identity with mouse neuronal-specific septin-3 and rat brain-specific G-septin. Alternative splicing leads to 3 SEP3 isoforms, SEP3A, SEP3B, and SEP3C, which encode proteins of 345, 337, and 71 amino acids, respectively. SEP3A and SEP3B contain a set of consensus sequence motifs for GTP binding, 2 predicted phosphorylation sites for PKG, and a conserved binding motif for SH3 domain-containing proteins.
Background: Neuronal-specific septin-3 belongs to the septin family of GTPases. Members of this family are required for cytokinesis. Expression is upregulated by retinoic acid in a human teratocarcinoma cell line. The specific function of this gene has not been determined. Alternative splicing of this gene results in multiple transcript variants. SEP3 shares almost 100% sequence identity with mouse neuronal-specific septin-3 and rat brain-specific G-septin. Alternative splicing leads to 3 SEP3 isoforms, SEP3A, SEP3B, and SEP3C, which encode proteins of 345, 337, and 71 amino acids, respectively. SEP3A and SEP3B contain a set of consensus sequence motifs for GTP binding, 2 predicted phosphorylation sites for PKG, and a conserved binding motif for SH3 domain-containing proteins.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Synonym Full Names
septin 3
NCBI Official Symbol
SEPTIN3
NCBI Official Synonym Symbols
SEPT3
NCBI Protein Information
neuronal-specific septin-3
UniProt Protein Name
Neuronal-specific septin-3
UniProt Gene Name
SEPT3
UniProt Entry Name
SEPT3_BOVIN
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Product Notes
The Human SEPT3 sept3 (Catalog #AAA331020) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331020 ELISA Kit recognizes Human SEPT3. It is sometimes possible for the material contained within the vial of "Neuronal-Specific Septin-3 (SEPT3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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