Standard Curve (Sample)
(Fig.1. Human Nuclear autoantigenic sperm protein (NASP) Standard Curve.)
Human Nuclear Autoantigenic Sperm Protein (NASP) ELISA Kit | NASP elisa kit
Human Nuclear Autoantigenic Sperm Protein (NASP) ELISA Kit
Gene Names
NASP; HMDRA1; FLB7527; PRO1999
Reactivity
Human
Synonyms
Nuclear Autoantigenic Sperm Protein (NASP); N/A; Human Nuclear Autoantigenic Sperm Protein (NASP) ELISA Kit; NASP; RP4-697E16.3; DKFZp547F162; FLB7527; FLJ31599; FLJ35510; MGC19722; MGC20372; MGC2297; PRO1999; N1/N2-like; histone H1-binding protein; nuclear autoantigenic sperm protein; NASP elisa kit
Reactivity
Human
Specificity
Human Nuclear autoantigenic sperm protein (NASP) ELISA Kit has high sensitivity and excellent specificity for detection of Human NASP. No significant cross-reactivity or interference between Human NASP and analogues was observed.
Sequence Length
788
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Nuclear autoantigenic sperm protein (NASP) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Nuclear autoantigenic sperm protein (NASP) Standard Curve.)
Related Product Information for NASP elisa kit
Description: This Human Nuclear autoantigenic sperm protein (NASP) ELISA Kit employs a two-site sandwich ELISA to quantitate NASP in samples. An antibody specific for NASP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NASP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NASP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NASP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: NASP encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa.
Background: NASP encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Nuclear autoantigenic sperm protein
NCBI Official Synonym Full Names
nuclear autoantigenic sperm protein
NCBI Official Symbol
NASP
NCBI Official Synonym Symbols
HMDRA1; FLB7527; PRO1999
NCBI Protein Information
nuclear autoantigenic sperm protein
UniProt Protein Name
Nuclear autoantigenic sperm protein
UniProt Gene Name
NASP
UniProt Synonym Gene Names
NASP
UniProt Entry Name
NASP_HUMAN
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Product Notes
The Human NASP nasp (Catalog #AAA331538) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331538 ELISA Kit recognizes Human NASP. It is sometimes possible for the material contained within the vial of "Nuclear Autoantigenic Sperm Protein (NASP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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