Standard Curve (Sample)
(Fig.1. Human Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform (PIK3CB) Standard Curve.)
Human Phosphatidylinositol-4, 5-bisphosphate 3-Kinase Catalytic Subunit beta Isoform (PIK3CB) ELISA Kit | PIK3CB elisa kit
Human Phosphatidylinositol-4, 5-bisphosphate 3-Kinase Catalytic Subunit beta Isoform (PIK3CB) ELISA Kit
Gene Names
PIK3CB; PI3K; PIK3C1; P110BETA; PI3KBETA
Reactivity
Human
Synonyms
Phosphatidylinositol-4, 5-bisphosphate 3-Kinase Catalytic Subunit beta Isoform (PIK3CB); N/A; Human Phosphatidylinositol-4, 5-bisphosphate 3-Kinase Catalytic Subunit beta Isoform (PIK3CB) ELISA Kit; PIK3CB; DKFZp779K1237; MGC133043; P110BETA; PI3K; PI3KBETA; PIK3C1; PI3-kinase p110 subunit beta; PtdIns-3-kinase p110; catalytic phosphatidylinositol 3-kinase beta; PIK3CB elisa kit
Reactivity
Human
Specificity
Human Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform (PIK3CB) ELISA Kit has high sensitivity and excellent specificity for detection of Human PIK3CB. No significant cross-reactivity or interference between Human PIK3CB and analogues was observed.
Sequence Length
582
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform (PIK3CB) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform (PIK3CB) Standard Curve.)
Related Product Information for PIK3CB elisa kit
Description: This Human Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform (PIK3CB) ELISA Kit employs a two-site sandwich ELISA to quantitate PIK3CB in samples. An antibody specific for PIK3CB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PIK3CB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIK3CB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIK3CB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. They have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kD catalytic subunit, such as PIK3CB, and an 85-kD adaptor subunit. The predicted 1,070-amino acid protein, called p110-beta by them, is 42% identical to that of bovine p110. Northern blot analysis revealed that the major 4.8-kb p110-beta transcript was expressed in several human and rodent cell lines, as well as in all mouse tissues tested. Minor larger transcripts were detected in some tissues and cell lines.
Background: Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. They have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kD catalytic subunit, such as PIK3CB, and an 85-kD adaptor subunit. The predicted 1,070-amino acid protein, called p110-beta by them, is 42% identical to that of bovine p110. Northern blot analysis revealed that the major 4.8-kb p110-beta transcript was expressed in several human and rodent cell lines, as well as in all mouse tissues tested. Minor larger transcripts were detected in some tissues and cell lines.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform isoform 2
NCBI Official Synonym Full Names
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta
NCBI Official Symbol
PIK3CB
NCBI Official Synonym Symbols
PI3K; PIK3C1; P110BETA; PI3KBETA
NCBI Protein Information
phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform
UniProt Protein Name
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform
UniProt Gene Name
PIK3CB
UniProt Synonym Gene Names
PIK3C1; PI3-kinase subunit beta; PI3K-beta; PI3Kbeta; PtdIns-3-kinase subunit beta; PtdIns-3-kinase subunit p110-beta; p110beta
UniProt Entry Name
PK3CB_HUMAN
Similar Products
Product Notes
The Human PIK3CB pik3cb (Catalog #AAA331388) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331388 ELISA Kit recognizes Human PIK3CB. It is sometimes possible for the material contained within the vial of "Phosphatidylinositol-4, 5-bisphosphate 3-Kinase Catalytic Subunit beta Isoform (PIK3CB), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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