IP (Immunoprecipitation)
(Immunoprecipitating PIK3CB in K562 whole cell lysateLane 1: Rabbit control IgG instead of in K562 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: K562 whole cell lysate?500ug?Lane 3: K562 whole cell lysate (10ug))
PIK3CB recombinant antibody
PIK3CB Antibody
Gene Names
PIK3CB; PI3K; PIK3C1; P110BETA; PI3KBETA
Reactivity
Human
Applications
Immunoprecipitation, Western Blot, ELISA
Purity
Affinity-chromatography
Synonyms
PIK3CB, Recombinant Antibody; PIK3CB Antibody; Phosphatidylinositol 4;5-bisphosphate 3-kinase catalytic subunit beta isoform (PI3-kinase subunit beta) (PI3K-beta) (PI3Kbeta) (PtdIns-3-kinase subunit beta) (EC 2.7.1.153) (Phosphatidylinositol 4;5-bisphosphate 3-kinase 110 kDa catalytic subunit beta) (PtdIns-3-kinase subunit p110-beta) (p110beta); PIK3CB; PIK3C1; PIK3CB recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
5A9
Purity/Purification
Affinity-chromatography
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, PH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for PIK3CB recombinant antibody
IP (Immunoprecipitation), WB (Western Blot), ELISA
Antibody Type
Recombinant Antibody
Conjugation
Non-conjugated
Immunogen
A synthesized peptide derived from human PI3 Kinase p110 beta
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
IP (Immunoprecipitation)
(Immunoprecipitating PIK3CB in K562 whole cell lysateLane 1: Rabbit control IgG instead of in K562 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: K562 whole cell lysate?500ug?Lane 3: K562 whole cell lysate (10ug))
IP (Immunoprecipitation)
(Immunoprecipitating PIK3CB in K562 whole cell lysateLane 1: Rabbit control IgG instead of in K562 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: K562 whole cell lysate?500ug?Lane 3: K562 whole cell lysate (10ug))
WB (Western Blot)
(Western BlotPositive WB detected in: Hela whole cell lysate, K562 whole cell lysateAll lanes: PIK3CB antibody at 1:1500SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 123 kDaObserved band size: 110 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: Hela whole cell lysate, K562 whole cell lysateAll lanes: PIK3CB antibody at 1:1500SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 123 kDaObserved band size: 110 kDa)
Related Product Information for PIK3CB recombinant antibody
Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (Phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors.
Product Categories/Family for PIK3CB recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
1070
NCBI Official Full Name
phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform isoform 2
NCBI Official Synonym Full Names
phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit beta
NCBI Official Symbol
PIK3CB
NCBI Official Synonym Symbols
PI3K; PIK3C1; P110BETA; PI3KBETA
NCBI Protein Information
phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform; PI3K-beta; PtdIns-3-kinase p110; PI3-kinase subunit beta; PI3-kinase p110 subunit beta; ptdIns-3-kinase subunit beta; ptdIns-3-kinase subunit p110-beta; phosphoinositide-3-kina
UniProt Protein Name
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform
UniProt Gene Name
PIK3CB
UniProt Synonym Gene Names
PIK3C1; PI3-kinase subunit beta; PI3K-beta; PI3Kbeta; PtdIns-3-kinase subunit beta; PtdIns-3-kinase subunit p110-beta; p110beta
UniProt Entry Name
PK3CB_HUMAN
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Product Notes
The PIK3CB pik3cb (Catalog #AAA243861) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PIK3CB Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PIK3CB can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the PIK3CB pik3cb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIK3CB, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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