Human Phosphoinositide-3-Kinase Catalytic Delta Polypeptide (PIK3Cd) ELISA Kit | PIK3Cd elisa kit

Human Phosphoinositide-3-Kinase Catalytic Delta Polypeptide (PIK3Cd) ELISA Kit

Cat. Num. AAA64895

• Gene Names: PIK3CD; APDS; PI3K; IMD14; p110D; P110DELTA
• Reactivity: Human
• Synonyms: Phosphoinositide-3-Kinase Catalytic Delta Polypeptide (PIK3Cd); N/A; Human Phosphoinositide-3-Kinase Catalytic Delta Polypeptide (PIK3Cd) ELISA Kit; p110D; P110δ; Phosphoinositide-3-Kinase C; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta; PIK3Cd elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PIK3Cd. No significant cross-reactivity or interference between PIK3Cd and analogues was observed.
• Sequence Length: 1044
• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.086ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PIK3Cd were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PIK3Cd were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Standard Curve (Sample)

Related Product Information for PIK3Cd elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PIK3Cd in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PIK3Cd. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PIK3Cd. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PIK3Cd, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PIK3Cd in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 156564405
• NCBI GeneID: 5293
• NCBI Accession #: NP_005017.3
• NCBI GenBank Nucleotide #: NM_005026.3
• UniProt Accession #: O00329; O15445; Q5SR49; A6NCG0; G1FFP1
• Molecular Weight: 33,107 Da
• NCBI Official Full Name: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform
• NCBI Official Synonym Full Names: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta
• NCBI Official Symbol: PIK3CD
• NCBI Official Synonym Symbols: APDS; PI3K; IMD14; p110D; P110DELTA
• NCBI Protein Information: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform
• UniProt Protein Name: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform
• UniProt Gene Name: PIK3CD
• UniProt Synonym Gene Names: PI3-kinase subunit delta; PI3K-delta; PI3Kdelta; PtdIns-3-kinase subunit delta; PtdIns-3-kinase subunit p110-delta; p110delta