Standard Curve (Sample)
Mouse phosphoinositide-3-kinase, catalytic, alpha polypeptide ELISA Kit | PIK3CA elisa kit
Mouse Phosphotylinosital 3 kinase, PI3K ELISA Kit
Gene Names
Pik3ca; p110; caPI3K; p110alpha; 6330412C24Rik
Reactivity
Mouse
Synonyms
phosphoinositide-3-kinase, catalytic, alpha polypeptide; N/A; Mouse Phosphotylinosital 3 kinase, PI3K ELISA Kit; Mouse Phosphotylinosital 3 kinase; PI3K ELISA Kit; MGC142161; MGC142163; PI3K; p110-alpha; PI3-kinase p110 subunit alpha; PtdIns-3-kinase p110; phosphatidylinositol 3-kinase; catalytic; 110-KD; alpha; alpha pol; phosphoinositide-3-kinase; alpha polypeptide; PIK3CA elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse PI3K. No significant cross-reactivity or interference between mouse PI3K and analogues was observed.
Sequence Length
1068
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
23.5 pg/ml -1500 pg/ml.
Sensitivity
The minimum detectable dose of mouse PI3K is typically less than 5.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for PIK3CA elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PI3K has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PI3K present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PI3K is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PI3K bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
124,412 Da
NCBI Official Full Name
phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
NCBI Official Synonym Full Names
phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
NCBI Official Symbol
Pik3ca
NCBI Official Synonym Symbols
p110; caPI3K; p110alpha; 6330412C24Rik
NCBI Protein Information
phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; PI3Kalpha; PI3K-alpha; PI3-kinase subunit alpha; ptdIns-3-kinase subunit alpha; ptdIns-3-kinase subunit p110-alpha; serine/threonine protein kinase PIK3CA; phosphoinositide-3-kinase catalytic alpha polypeptide; phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
UniProt Protein Name
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
UniProt Gene Name
Pik3ca
UniProt Synonym Gene Names
PI3-kinase subunit alpha; PI3K-alpha; PI3Kalpha; PtdIns-3-kinase subunit alpha; PtdIns-3-kinase subunit p110-alpha; p110alpha
UniProt Entry Name
PK3CA_MOUSE
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Product Notes
The Mouse PIK3CA pik3ca (Catalog #AAA80349) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA80349 ELISA Kit recognizes Mouse PIK3CA. It is sometimes possible for the material contained within the vial of "phosphoinositide-3-kinase, catalytic, alpha polypeptide, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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