Reactivity
Human
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids
Detection Range
0.5-150ng/ml
Sensitivity
0.33ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Related Product Information for pNF-H elisa kit
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human pNF-H antibody. pNF-H present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human pNF-H Antibody is added and binds to pNF-H in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated pNF-H antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human pNF-H. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Phosphorylated Neurofilament (also known as pNF-H) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Phosphorylated Neurofilament (also known as pNF-H) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.