Standard Curve (Sample)
(Fig.1. Human Pituitary adenylate cyclase activating polypeptide (PACAP) Standard Curve.)
Human Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) ELISA Kit | PACAP elisa kit
Human Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) ELISA Kit
Gene Names
ADCYAP1; PACAP
Reactivity
Human
Synonyms
Pituitary Adenylate Cyclase Activating Polypeptide (PACAP); N/A; Human Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) ELISA Kit; ADCYAP1; MGC126852; PACAP; OTTHUMP00000162201; adenylate cyclase activating polypeptide; PACAP elisa kit
Reactivity
Human
Specificity
Human Pituitary adenylate cyclase activating polypeptide (PACAP) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADCYAP1. No significant cross-reactivity or interference between Human ADCYAP1 and analogues was observed.
Sequence Length
176
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Pituitary adenylate cyclase activating polypeptide (PACAP) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Pituitary adenylate cyclase activating polypeptide (PACAP) Standard Curve.)
Related Product Information for PACAP elisa kit
Description: This Human Pituitary adenylate cyclase activating polypeptide (PACAP) ELISA Kit employs a two-site sandwich ELISA to quantitate ADCYAP1 in samples. An antibody specific for ADCYAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADCYAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADCYAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADCYAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Pituitary adenylate cyclase-activating peptide, adenylate cyclase-activating polypeptide 1. Mediated by adenylate cyclase-activating polypeptide 1 receptors, this polypeptide stimulates adenylate cyclase and subsequently increases the cAMP level in target cells. Adenylate cyclase-activating polypeptide 1 not only is a hypophysiotropic hormone but also functions as a neurotransmitter and neuromodulator. In addition, it plays a role in paracrine and autocrine regulation of certain types of cells. This gene is composed of five exons. Exons 1 and 2 encode the 5' UTR and signal peptide, respectively; exon 4 encodes an adenylate cyclase-activating polypeptide 1-related peptide; and exon 5 encodes the mature peptide and 3' UTR.
Background: Pituitary adenylate cyclase-activating peptide, adenylate cyclase-activating polypeptide 1. Mediated by adenylate cyclase-activating polypeptide 1 receptors, this polypeptide stimulates adenylate cyclase and subsequently increases the cAMP level in target cells. Adenylate cyclase-activating polypeptide 1 not only is a hypophysiotropic hormone but also functions as a neurotransmitter and neuromodulator. In addition, it plays a role in paracrine and autocrine regulation of certain types of cells. This gene is composed of five exons. Exons 1 and 2 encode the 5' UTR and signal peptide, respectively; exon 4 encodes an adenylate cyclase-activating polypeptide 1-related peptide; and exon 5 encodes the mature peptide and 3' UTR.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
PACAP
NCBI Official Synonym Full Names
adenylate cyclase activating polypeptide 1
NCBI Official Symbol
ADCYAP1
NCBI Official Synonym Symbols
PACAP
NCBI Protein Information
pituitary adenylate cyclase-activating polypeptide
UniProt Protein Name
Pituitary adenylate cyclase-activating polypeptide
UniProt Gene Name
ADCYAP1
UniProt Synonym Gene Names
PACAP; PACAP-27; PACAP27; PACAP-38; PACAP38
UniProt Entry Name
PACA_HUMAN
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Product Notes
The Human PACAP adcyap1 (Catalog #AAA331122) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331122 ELISA Kit recognizes Human PACAP. It is sometimes possible for the material contained within the vial of "Pituitary Adenylate Cyclase Activating Polypeptide (PACAP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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