Standard Curve (Sample)
(Human PLAT/TPA PicoKine ELISA Kit standard curve)
Human PLAT/TPA ELISA Kit | PLAT elisa kit
Human PLAT/TPA PicoKine ELISA Kit
Gene Names
PLAT; TPA; T-PA
Reactivity
Human
Synonyms
PLAT/TPA; N/A; Human PLAT/TPA PicoKine ELISA Kit; Tissue-type plasminogen activator; plasminogen activator, tissue; Alteplase; Plasminogen activator tissue; Plasminogen activator tissue type; PLAT; Reteplase; t-PA; T-plasminogen activator; Tissue-type plasminogen activator chain B; tPA; TPA_HUMAN; PLAT elisa kit
Reactivity
Human
Specificity
Natural and recombinant human PLAT
Sequence Length
562
Samples
Cell culture supernates, serum, plasma (heparin, EDTA, citrate) and urine
Assay Type
Sandwich
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
(Human PLAT/TPA PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human PLAT/TPA PicoKine ELISA Kit standard curve)
Related Product Information for PLAT elisa kit
Principle of the assay: human PLAT ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PLAT has been precoated onto 96-well plates. Standards(CHO, M1-P562) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PLAT is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PLAT amount of sample captured in plate.
Background: Plasminogen activator, tissue, also called PLAT or TPA is a serine protease. This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This gene was mapped to 8p11.21. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism.
Background: Plasminogen activator, tissue, also called PLAT or TPA is a serine protease. This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This gene was mapped to 8p11.21. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
44,373 Da
NCBI Official Full Name
Tissue-type plasminogen activator
NCBI Official Synonym Full Names
plasminogen activator, tissue
NCBI Official Symbol
PLAT
NCBI Official Synonym Symbols
TPA; T-PA
NCBI Protein Information
tissue-type plasminogen activator; alteplase; reteplase; t-plasminogen activator; plasminogen activator, tissue type; tissue plasminogen activator (t-PA)
UniProt Protein Name
Tissue-type plasminogen activator
UniProt Gene Name
PLAT
UniProt Synonym Gene Names
t-PA; t-plasminogen activator; tPA
UniProt Entry Name
TPA_HUMAN
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Product Notes
The Human PLAT plat (Catalog #AAA46009) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA46009 ELISA Kit recognizes Human PLAT. It is sometimes possible for the material contained within the vial of "PLAT/TPA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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