Standard Curve (Sample)
General tPA functional assay ELISA Kit | PLAT elisa kit
Active human tPA functional assay ELISA Kit
Gene Names
PLAT; TPA; T-PA
Reactivity
General
Synonyms
tPA functional assay; N/A; Active human tPA functional assay ELISA Kit; PLAT elisa kit
Reactivity
General
Specificity
These studies are currently in progress. Please contact us for more information.
Form/Format
Complete Kit
Sequence Length
562
Samples
Human plasma and other biological fluids
Sensitivity
Sensitivity was calculated by determining the OD of 20 reps of So and 20 reps of the low standard. Sensitivity was determined to be 0.006 IU/ml.
Intra-assay Precision
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision
These studies are currently in progress. Please contact us for more information.
Preparation and Storage
Store at 4 degree C. Shelf Life: 12 months from manufacture, see label for expiration date
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for PLAT elisa kit
Intended Uses: This human tPA activity assay is intended for the quantitative determination of active tissue plasminogen activator in human plasma and other biological fluids. For research use only.
Principle of the Assay: Functionally active tPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. After appropriate washing steps, polyclonal anti-human tPA primary antibody binds to the captured tPA. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB is used for color development at 450nm. The amount of color development is directly proportional to the concentration of active tPA in the sample.
Background: Tissue plasminogen activator is a serine protease that catalyzes the activation of plasminogen to plasmin [1]. Clinical studies have indicated that high tPA levels may increase the risk for thrombosis [2], whereas decreased levels may cause neuronal plasticity and degeneration [3].
Principle of the Assay: Functionally active tPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. After appropriate washing steps, polyclonal anti-human tPA primary antibody binds to the captured tPA. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB is used for color development at 450nm. The amount of color development is directly proportional to the concentration of active tPA in the sample.
Background: Tissue plasminogen activator is a serine protease that catalyzes the activation of plasminogen to plasmin [1]. Clinical studies have indicated that high tPA levels may increase the risk for thrombosis [2], whereas decreased levels may cause neuronal plasticity and degeneration [3].
Product Categories/Family for PLAT elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62,917 Da
NCBI Official Full Name
tissue-type plasminogen activator isoform 1 preproprotein
NCBI Official Synonym Full Names
plasminogen activator, tissue
NCBI Official Symbol
PLAT
NCBI Official Synonym Symbols
TPA; T-PA
NCBI Protein Information
tissue-type plasminogen activator; alteplase; reteplase; t-plasminogen activator; plasminogen activator, tissue type; tissue plasminogen activator (t-PA)
UniProt Protein Name
Tissue-type plasminogen activator
UniProt Gene Name
PLAT
UniProt Synonym Gene Names
t-PA; t-plasminogen activator; tPA
UniProt Entry Name
TPA_HUMAN
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Product Notes
The General PLAT plat (Catalog #AAA37754) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA37754 ELISA Kit recognizes General PLAT. It is sometimes possible for the material contained within the vial of "tPA functional assay, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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