Standard Curve (Sample)
(Fig.1. Human Poliovirus receptor-related protein 4 (PVRL4) Standard Curve.)
Human Poliovirus Receptor-Related Protein 4 (PVRL4) ELISA Kit | PVRL4 elisa kit
Human Poliovirus Receptor-Related Protein 4 (PVRL4) ELISA Kit
Gene Names
NECTIN4; LNIR; PRR4; EDSS1; PVRL4; nectin-4
Reactivity
Human
Synonyms
Poliovirus Receptor-Related Protein 4 (PVRL4); N/A; Human Poliovirus Receptor-Related Protein 4 (PVRL4) ELISA Kit; PVRL4; LNIR; PRR4; nectin-4; Ig superfamily receptor LNIR; nectin 4; PVRL4 elisa kit
Reactivity
Human
Specificity
Human Poliovirus receptor-related protein 4 (PVRL4) ELISA Kit has high sensitivity and excellent specificity for detection of Human PVRL4. No significant cross-reactivity or interference between Human PVRL4 and analogues was observed.
Sequence Length
510
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Poliovirus receptor-related protein 4 (PVRL4) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Poliovirus receptor-related protein 4 (PVRL4) Standard Curve.)
Related Product Information for PVRL4 elisa kit
Description: This Human Poliovirus receptor-related protein 4 (PVRL4) ELISA Kit employs a two-site sandwich ELISA to quantitate PVRL4 in samples. An antibody specific for PVRL4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PVRL4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PVRL4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PVRL4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Poliovirus receptor-like proteins (PVRLs), such as PVRL4, are adhesion receptors of the immunoglobulin superfamily and function in cell-cell adhesion (Reymond et al., 2001). By database analysis, Reymond et al. (2001) identified the PVRL4 gene, which encodes a 510-amino acid protein with a predicted molecular mass of 55.5 kD. It contains 1 predicted transmembrane domain, followed by a 139-residue cytoplasmic sequence. Human and murine PVRL4 share 92% amino acid identity. Northern blot analysis showed strong expression of a 3.7-kb human PVRL4 transcript in placenta and weak expression in trachea. Murine Pvrl4 was expressed at embryonic days 11, 15 and 17. Immunohistochemistry revealed human PVRL4 localized to endothelial cells from vessels in placental villi.
Background: Poliovirus receptor-like proteins (PVRLs), such as PVRL4, are adhesion receptors of the immunoglobulin superfamily and function in cell-cell adhesion (Reymond et al., 2001). By database analysis, Reymond et al. (2001) identified the PVRL4 gene, which encodes a 510-amino acid protein with a predicted molecular mass of 55.5 kD. It contains 1 predicted transmembrane domain, followed by a 139-residue cytoplasmic sequence. Human and murine PVRL4 share 92% amino acid identity. Northern blot analysis showed strong expression of a 3.7-kb human PVRL4 transcript in placenta and weak expression in trachea. Murine Pvrl4 was expressed at embryonic days 11, 15 and 17. Immunohistochemistry revealed human PVRL4 localized to endothelial cells from vessels in placental villi.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
nectin-4
NCBI Official Synonym Full Names
nectin cell adhesion molecule 4
NCBI Official Symbol
NECTIN4
NCBI Official Synonym Symbols
LNIR; PRR4; EDSS1; PVRL4; nectin-4
NCBI Protein Information
nectin-4
UniProt Protein Name
Poliovirus receptor-related protein 4
UniProt Gene Name
PVRL4
UniProt Synonym Gene Names
LNIR; PRR4
UniProt Entry Name
PVRL4_HUMAN
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Product Notes
The Human PVRL4 pvrl4 (Catalog #AAA331217) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331217 ELISA Kit recognizes Human PVRL4. It is sometimes possible for the material contained within the vial of "Poliovirus Receptor-Related Protein 4 (PVRL4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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