Standard Curve (Sample)
(Fig.1. Human Poly (ADP-ribose) glycohydrolase (PARG) Standard Curve.)
Human Poly (ADP-Ribose) Glycohydrolase (PARG) ELISA Kit | PARG elisa kit
Human Poly (ADP-Ribose) Glycohydrolase (PARG) ELISA Kit
Gene Names
BPHL; MCNAA; BPH-RP; VACVASE
Reactivity
Human
Synonyms
Poly (ADP-Ribose) Glycohydrolase (PARG); N/A; Human Poly (ADP-Ribose) Glycohydrolase (PARG) ELISA Kit; PARG; FLJ60456; PARG99; poly(ADP-ribose) glycohydrolase 60 kDa isoform; PARG elisa kit
Reactivity
Human
Specificity
Human Poly (ADP-ribose) glycohydrolase (PARG) ELISA Kit has high sensitivity and excellent specificity for detection of Human PARG. No significant cross-reactivity or interference between Human PARG and analogues was observed.
Sequence Length
274
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Poly (ADP-ribose) glycohydrolase (PARG) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Poly (ADP-ribose) glycohydrolase (PARG) Standard Curve.)
Related Product Information for PARG elisa kit
Description: This Human Poly (ADP-ribose) glycohydrolase (PARG) ELISA Kit employs a two-site sandwich ELISA to quantitate PARG in samples. An antibody specific for PARG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PARG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PARG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PARG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PARG is a protein involved in a number of cellular processes involving mainly DNA repair and programmed cell death.RP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like caspases in vitroand is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP1 is a chromatin-associated enzyme, poly-ADP ribose polymerase-1, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. PARP1 has a role in repair of single-stranded DNA (ssDNA) breaks.
Background: PARG is a protein involved in a number of cellular processes involving mainly DNA repair and programmed cell death.RP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like caspases in vitroand is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP1 is a chromatin-associated enzyme, poly-ADP ribose polymerase-1, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. PARP1 has a role in repair of single-stranded DNA (ssDNA) breaks.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
valacyclovir hydrolase isoform 2
NCBI Official Synonym Full Names
biphenyl hydrolase like
NCBI Official Symbol
BPHL
NCBI Official Synonym Symbols
MCNAA; BPH-RP; VACVASE
NCBI Protein Information
valacyclovir hydrolase
UniProt Protein Name
Poly(ADP-ribose) glycohydrolase
UniProt Gene Name
PARG
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Product Notes
The Human PARG parg (Catalog #AAA331471) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331471 ELISA Kit recognizes Human PARG. It is sometimes possible for the material contained within the vial of "Poly (ADP-Ribose) Glycohydrolase (PARG), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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