Standard Curve (Sample)
(Fig.1. Human Polymerase I and transcript release factor (PTRF) Standard Curve.)
Human Polymerase I And Transcript Release Factor (PTRF) ELISA Kit | PTRF elisa kit
Human Polymerase I And Transcript Release Factor (PTRF) ELISA Kit
Gene Names
CAVIN1; CGL4; PTRF; CAVIN; FKSG13; cavin-1
Reactivity
Human
Synonyms
Polymerase I And Transcript Release Factor (PTRF); N/A; Human Polymerase I And Transcript Release Factor (PTRF) ELISA Kit; PTRF; FKSG13; FLJ90031; cavin-1; RNA polymerase I and transcript release factor; TTF-I interacting peptide 12; PTRF elisa kit
Reactivity
Human
Specificity
Human Polymerase I and transcript release factor (PTRF) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTRF. No significant cross-reactivity or interference between Human PTRF and analogues was observed.
Sequence Length
390
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Polymerase I and transcript release factor (PTRF) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Polymerase I and transcript release factor (PTRF) Standard Curve.)
Related Product Information for PTRF elisa kit
Description: This Human Polymerase I and transcript release factor (PTRF) ELISA Kit employs a two-site sandwich ELISA to quantitate PTRF in samples. An antibody specific for PTRF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTRF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTRF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTRF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PTRF encodes a protein that enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts. This protein regulates rRNA transcription by promoting the dissociation of transcription complexes and the reinitiation of polymerase I on nascent rRNA transcripts. This protein also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins. This protein translocates from caveolae to the cytoplasm after insulin stimulation. Caveolae contain truncated forms of this protein and may be the site of phosphorylation-dependent proteolysis. This protein is also thought to modify lipid metabolism and insulin-regulated gene expression.
Background: PTRF encodes a protein that enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts. This protein regulates rRNA transcription by promoting the dissociation of transcription complexes and the reinitiation of polymerase I on nascent rRNA transcripts. This protein also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins. This protein translocates from caveolae to the cytoplasm after insulin stimulation. Caveolae contain truncated forms of this protein and may be the site of phosphorylation-dependent proteolysis. This protein is also thought to modify lipid metabolism and insulin-regulated gene expression.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Caveolae-associated protein 1
NCBI Official Synonym Full Names
caveolae associated protein 1
NCBI Official Symbol
CAVIN1
NCBI Official Synonym Symbols
CGL4; PTRF; CAVIN; FKSG13; cavin-1
NCBI Protein Information
caveolae-associated protein 1
UniProt Protein Name
Polymerase I and transcript release factor
UniProt Gene Name
PTRF
UniProt Entry Name
PTRF_HUMAN
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Product Notes
The Human PTRF ptrf (Catalog #AAA331226) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331226 ELISA Kit recognizes Human PTRF. It is sometimes possible for the material contained within the vial of "Polymerase I And Transcript Release Factor (PTRF), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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