Human Polyserase-2 (PRSS36) ELISA Kit | PRSS36 elisa kit

Human Polyserase-2 (PRSS36) ELISA Kit

Cat. Num. AAA331284

• Reactivity: Human
• Synonyms: Polyserase-2 (PRSS36); N/A; Human Polyserase-2 (PRSS36) ELISA Kit; PRSS36; FLJ90661; polyserase-2; polyserine protease-2; PRSS36 elisa kit

• Reactivity: Human
• Specificity: Human Polyserase-2 (PRSS36) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRSS36. No significant cross-reactivity or interference between Human PRSS36 and analogues was observed.
• Sequence Length: 850
• Assay Type: Sandwich
• Samples: Human serum, cell culture supernates and other biological fluids
• Detection Range: 3.25 pg/ml - 60 pg/ml
• Sensitivity: < 0.1pg/ml

• Intra-assay Precision: Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?9%.
• Inter-assay Precision: Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?11%.
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for PRSS36 elisa kit

Background: The deduced 855-amino acid protein has a predicted molecular mass of 93 kD and contains 9 potential N-glycosylation sites. PRSS36 contains a signal peptide, followed by a propeptide domain, an active serine protease domain with the catalytic his, asp, and ser residues, and 2 serine protease domains predicted to be catalytically inactive. The first serine protease domain shares amino acid identity of 40 to 42% with gamma-1 tryptase (TPSG1), pancreasin, matriptase (ST14;), matriptase-2 (TMPRSS6), and polyserase-1 (TMPRSS9). Northern blot analysis detected a 5-kb transcript in fetal kidney and adult skeletal muscle, liver, placenta,

Principle of the Assay: Human Polyserase-2 (PRSS36) ELISA Kit employs a two-site sandwich ELISA to quantitate PRSS36 in samples. An antibody specific for PRSS36 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRSS36 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human PRSS36 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of PRSS36 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 385137133
• NCBI GeneID: 146547
• NCBI Accession #: NP_001245219.1
• NCBI GenBank Nucleotide #: NP_001245219.1
• UniProt Accession #: Q5K4E3
• NCBI Official Full Name: polyserase-2 isoform 2
• NCBI Official Synonym Full Names: serine protease 36
• NCBI Official Symbol: PRSS36
• NCBI Protein Information: polyserase-2
• UniProt Protein Name: Polyserase-2
• UniProt Gene Name: PRSS36