Standard Curve (Sample)
(Fig.1. Human Probable asparaginyl-tRNA synthetase, mitochondrial (NARS2) Standard Curve.)
Human Probable Asparaginyl-tRNA Synthetase, Mitochondrial (NARS2) ELISA Kit | NARS2 elisa kit
Human Probable Asparaginyl-tRNA Synthetase, Mitochondrial (NARS2) ELISA Kit
Gene Names
NARS2; SLM5; asnRS; DFNB94
Reactivity
Human
Synonyms
Probable Asparaginyl-tRNA Synthetase, Mitochondrial (NARS2); N/A; Human Probable Asparaginyl-tRNA Synthetase, Mitochondrial (NARS2) ELISA Kit; NARS2; FLJ23441; SLM5; asparagine tRNA ligase 2; mitochondrial (putative); asparaginyl-tRNA synthetase 2; mitochondrial; NARS2 elisa kit
Reactivity
Human
Specificity
Human Probable asparaginyl-tRNA synthetase, mitochondrial (NARS2) ELISA Kit has high sensitivity and excellent specificity for detection of Human NARS2. No significant cross-reactivity or interference between Human NARS2 and analogues was observed.
Sequence Length
477
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Probable asparaginyl-tRNA synthetase, mitochondrial (NARS2) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Probable asparaginyl-tRNA synthetase, mitochondrial (NARS2) Standard Curve.)
Related Product Information for NARS2 elisa kit
Description: This Human Probable asparaginyl-tRNA synthetase, mitochondrial (NARS2) ELISA Kit employs a two-site sandwich ELISA to quantitate NARS2 in samples. An antibody specific for NARS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NARS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NARS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NARS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: NARS2 encodes a putative member of the class II family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of asparagine to tRNA molecules. Mutations in NARS2 have been associated with combined oxidative phosphorylation deficiency 24 (COXPD24).
Background: NARS2 encodes a putative member of the class II family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of asparagine to tRNA molecules. Mutations in NARS2 have been associated with combined oxidative phosphorylation deficiency 24 (COXPD24).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
probable asparagine--tRNA ligase, mitochondrial isoform 1
NCBI Official Synonym Full Names
asparaginyl-tRNA synthetase 2, mitochondrial
NCBI Official Symbol
NARS2
NCBI Official Synonym Symbols
SLM5; asnRS; DFNB94
NCBI Protein Information
probable asparagine--tRNA ligase, mitochondrial
UniProt Protein Name
Probable asparagine--tRNA ligase, mitochondrial
UniProt Gene Name
NARS2
UniProt Synonym Gene Names
AsnRS
UniProt Entry Name
SYNM_HUMAN
Similar Products
Product Notes
The Human NARS2 nars2 (Catalog #AAA331539) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331539 ELISA Kit recognizes Human NARS2. It is sometimes possible for the material contained within the vial of "Probable Asparaginyl-tRNA Synthetase, Mitochondrial (NARS2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.