Rabbit anti-Human NARS2 Polyclonal Antibody | anti-NARS2 antibody

Anti-NARS2 Antibody, Rabbit Polyclonal

Cat. Num. AAA255991

• Reactivity: Human
• Applications: Immunoprecipitation, Immunohistochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: NARS2, Antibody; Anti-NARS2 Antibody, Rabbit Polyclonal; NARS2 Antibody, Rabbit PAb, Antigen Affinity Purified; asparaginyl-tRNA synthetase 2, mitochondrial (putative); Anti-asnRS Antibody; Anti-SLM5 Antibody; anti-NARS2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human NARS2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-NARS2 antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: E Coli-derived Human NARS2 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human NARS2 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(NARS2 was immunoprecipitated using:Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-NARS2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-NARS2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 54 kDaObserved band size :54 kDa)

IHC (Immunohistochemisry)

(Immunochemical staining of human NARS2 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohiostchemistry)

(Immunochemical staining of human NARS2 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot)

(Anti-NARS2 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: U937 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:54 kDaObserved band size:54 kDa)

Related Product Information for anti-NARS2 antibody

NARS2 (Asparaginyl-TRNA Synthetase 2, Mitochondrial) is a Protein Coding gene. The NARS2 gene, located on 11q14.1, is conserved in chimpanzee, Rhesus monkey, dog, and cow, etc. NARS2 belongs to the class-II aminoacyl-tRNA synthetase family. This gene encodes a putative member of the class II family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of asparagine to tRNA molecules. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency 24 (COXPD24). NARS2 mutations cause various clinical phenotypes, including non-syndromic hearing loss, Leigh syndrome, intellectual disability with epilepsy, and severe myopathy.

References

Mizuguchi T, et al. (2017) Erratum: Pars2 and nars2 mutations in infantile-onset neurodegenerative disorder. J Hum Genet 62 (5): 587.Liu G, et al. (2018) Alzheimer's disease risk variant rs2373115 regulates gab2 and nars2 expression in human brain tissues. J Mol Neurosci 66 (1): 37-43.Bruwer Z, et al. (2018) Inborn errors of metabolism in a cohort of pregnancies with non-immune hydrops fetalis: A single center experience. J Perinat Med 46 (9): 968-974.