Standard Curve (Sample)
(Fig.1. Human Programmed cell death 1 ligand 2 (PDCD1LG2) Standard Curve.)
Human Programmed Cell Death 1 Ligand 2 (PDCD1LG2) ELISA Kit | PDCD1LG2 elisa kit
Human Programmed Cell Death 1 Ligand 2 (PDCD1LG2) ELISA Kit
Gene Names
PDCD1LG2; B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2
Reactivity
Human
Synonyms
Programmed Cell Death 1 Ligand 2 (PDCD1LG2); N/A; Human Programmed Cell Death 1 Ligand 2 (PDCD1LG2) ELISA Kit; PDCD1LG2; B7DC; Btdc; CD273; MGC142238; MGC142240; PD-L2; PDCD1L2; PDL2; bA574F11.2; PD-1-ligand 2; programmed death ligand 2; PDCD1LG2 elisa kit
Reactivity
Human
Specificity
Human Programmed cell death 1 ligand 2 (PDCD1LG2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PDCD1LG2. No significant cross-reactivity or interference between Human PDCD1LG2 and analogues was observed.
Sequence Length
273
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Programmed cell death 1 ligand 2 (PDCD1LG2) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Programmed cell death 1 ligand 2 (PDCD1LG2) Standard Curve.)
Related Product Information for PDCD1LG2 elisa kit
Description: This Human Programmed cell death 1 ligand 2 (PDCD1LG2) ELISA Kit employs a two-site sandwich ELISA to quantitate PDCD1LG2 in samples. An antibody specific for PDCD1LG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDCD1LG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDCD1LG2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDCD1LG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Using flow cytometric analysis, Latchman et al. (2001) showed that the IgV region of mouse Pdl2, like Pdl1, binds to human PDCD1 but not to CTLA4, CD28, or ICOS. Stimulation of mouse T cells with anti-CD3 and Pdl2 or Pdl1 resulted in inhibition of proliferation and cytokine production, and Pdl2 could inhibit T-cell receptor -CD28 signals at low antigen concentrations. Latchman et al. (2001) proposed that blocking of the PDL-PDCD1 pathway may enhance antitumor immunity, whereas stimulating this pathway may help downregulate graft rejection and autoimmune and allergic reactions. Latchman et al. (2001) mapped the PDL2 gene to chromosome 9p24.2, in close proximity to the PDL1 gene. Tseng et al. (2001) mapped the mouse Pdl2 gene to chromosome 19C2-C3.
Background: Using flow cytometric analysis, Latchman et al. (2001) showed that the IgV region of mouse Pdl2, like Pdl1, binds to human PDCD1 but not to CTLA4, CD28, or ICOS. Stimulation of mouse T cells with anti-CD3 and Pdl2 or Pdl1 resulted in inhibition of proliferation and cytokine production, and Pdl2 could inhibit T-cell receptor -CD28 signals at low antigen concentrations. Latchman et al. (2001) proposed that blocking of the PDL-PDCD1 pathway may enhance antitumor immunity, whereas stimulating this pathway may help downregulate graft rejection and autoimmune and allergic reactions. Latchman et al. (2001) mapped the PDL2 gene to chromosome 9p24.2, in close proximity to the PDL1 gene. Tseng et al. (2001) mapped the mouse Pdl2 gene to chromosome 19C2-C3.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
programmed cell death 1 ligand 2
NCBI Official Synonym Full Names
programmed cell death 1 ligand 2
NCBI Official Symbol
PDCD1LG2
NCBI Official Synonym Symbols
B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2
NCBI Protein Information
programmed cell death 1 ligand 2
UniProt Protein Name
Programmed cell death 1 ligand 2
UniProt Gene Name
PDCD1LG2
UniProt Synonym Gene Names
B7DC; CD273; PDCD1L2; PDL2; PD-1 ligand 2; PD-L2; PDCD1 ligand 2; Programmed death ligand 2; B7-DC
UniProt Entry Name
PD1L2_HUMAN
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Product Notes
The Human PDCD1LG2 pdcd1lg2 (Catalog #AAA331447) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331447 ELISA Kit recognizes Human PDCD1LG2. It is sometimes possible for the material contained within the vial of "Programmed Cell Death 1 Ligand 2 (PDCD1LG2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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