Human Prosaposin (PSAP) ELISA Kit | PSAP elisa kit

Human Prosaposin (PSAP) ELISA Kit

Cat. Num. AAA331275

• Gene Names: PSAP; GLBA; SAP1
• Reactivity: Human
• Synonyms: Prosaposin (PSAP); N/A; Human Prosaposin (PSAP) ELISA Kit; PSAP; FLJ00245; GLBA; MGC110993; SAP1; sphingolipid activator protein-1; PSAP elisa kit

• Reactivity: Human
• Specificity: Human Prosaposin (PSAP) ELISA Kit has high sensitivity and excellent specificity for detection of Human PSAP. No significant cross-reactivity or interference between Human PSAP and analogues was observed.
• Sequence Length: 527
• Assay Type: Sandwich
• Samples: Human serum, cell culture supernates and other biological fluids
• Detection Range: 50 ng/L - 800 ng/L
• Sensitivity: < 5ng/L

• Intra-assay Precision: Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?9%.
• Inter-assay Precision: Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?11%.
• Preparation and Storage: Store at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for PSAP elisa kit

Background: Prosaposin also known as PSAP is a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Saposin is an acronym for Sphingolipid Activator PrO[S]teINs. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities.Saposins A-D are required for the hydrolysis of certain shingolipids by specific lysosomal hydrolases.

Principle of the Assay: Human Prosaposin (PSAP) ELISA Kit employs a two-site sandwich ELISA to quantitate PSAP in samples. An antibody specific for PSAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PSAP present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human PSAP detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of PSAP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 110224476
• NCBI GeneID: 5660
• NCBI Accession #: NP_001035930.1
• NCBI GenBank Nucleotide #: NP_001035930.1
• UniProt Accession #: P07602
• NCBI Official Full Name: prosaposin isoform b preproprotein
• NCBI Official Synonym Full Names: prosaposin
• NCBI Official Symbol: PSAP
• NCBI Official Synonym Symbols: GLBA; SAP1
• NCBI Protein Information: prosaposin
• UniProt Protein Name: Proactivator polypeptide
• UniProt Gene Name: PSAP
• UniProt Synonym Gene Names: GLBA; SAP1; CSAct; SAP-1; SAP-2
• UniProt Entry Name: SAP_HUMAN