Standard Curve (Sample)
(Fig.1. Human Proteasome subunit beta type-6 (PSMB6) Standard Curve.)
Human Proteasome Subunit beta Type-6 (PSMB6) ELISA Kit | PSMB6 elisa kit
Human Proteasome Subunit beta Type-6 (PSMB6) ELISA Kit
Gene Names
PSMB6; Y; LMPY; DELTA
Reactivity
Human
Synonyms
Proteasome Subunit beta Type-6 (PSMB6); N/A; Human Proteasome Subunit beta Type-6 (PSMB6) ELISA Kit; PSMB6; DELTA; LMPY; MGC5169; Y; PSY large multifunctional protease Y; macropain delta chain; multicatalytic endopeptidase complex delta chain; proteasome beta 6 subunit; proteasome catalytic subunit 1; proteaso; PSMB6 elisa kit
Reactivity
Human
Specificity
Human Proteasome subunit beta type-6 (PSMB6) ELISA Kit has high sensitivity and excellent specificity for detection of Human PSMB6. No significant cross-reactivity or interference between Human PSMB6 and analogues was observed.
Sequence Length
239
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Proteasome subunit beta type-6 (PSMB6) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Proteasome subunit beta type-6 (PSMB6) Standard Curve.)
Related Product Information for PSMB6 elisa kit
Description: This Human Proteasome subunit beta type-6 (PSMB6) ELISA Kit employs a two-site sandwich ELISA to quantitate PSMB6 in samples. An antibody specific for PSMB6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PSMB6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSMB6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSMB6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides.PSMB6encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 1i (proteasome beta 9 subunit).
Background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides.PSMB6encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 1i (proteasome beta 9 subunit).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Proteasome subunit beta type-6
NCBI Official Synonym Full Names
proteasome subunit beta 6
NCBI Official Symbol
PSMB6
NCBI Official Synonym Symbols
Y; LMPY; DELTA
NCBI Protein Information
proteasome subunit beta type-6
UniProt Protein Name
Proteasome subunit beta type-6
UniProt Gene Name
PSMB6
UniProt Synonym Gene Names
LMPY; Y
UniProt Entry Name
PSB6_HUMAN
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Product Notes
The Human PSMB6 psmb6 (Catalog #AAA331264) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331264 ELISA Kit recognizes Human PSMB6. It is sometimes possible for the material contained within the vial of "Proteasome Subunit beta Type-6 (PSMB6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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