Standard Curve (Sample)
(Fig.1. Human Pyrin domain-containing protein 1 (PYDC1) Standard Curve.)
Human Pyrin Domain-Containing Protein 1 (PYDC1) ELISA Kit | PYDC1 elisa kit
Human Pyrin Domain-Containing Protein 1 (PYDC1) ELISA Kit
Gene Names
PYDC1; ASC2; POP1; PYC1; cPOP1
Reactivity
Human
Synonyms
Pyrin Domain-Containing Protein 1 (PYDC1); N/A; Human Pyrin Domain-Containing Protein 1 (PYDC1) ELISA Kit; PYDC1; ASC2; POP1; PYC1; PAAD-only protein; pyrin domain containing 1; pyrin-domain containing protein 1; pyrin-only protein 1; PYDC1 elisa kit
Reactivity
Human
Specificity
Human Pyrin domain-containing protein 1 (PYDC1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PYDC1. No significant cross-reactivity or interference between Human PYDC1 and analogues was observed.
Sequence Length
89
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Pyrin domain-containing protein 1 (PYDC1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Pyrin domain-containing protein 1 (PYDC1) Standard Curve.)
Related Product Information for PYDC1 elisa kit
Description: This Human Pyrin domain-containing protein 1 (PYDC1) ELISA Kit employs a two-site sandwich ELISA to quantitate PYDC1 in samples. An antibody specific for PYDC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PYDC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PYDC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYDC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: ASC2, a PYD-only protein that functions as a modulator of multidomain PYD-containing proteins involved in NF-kappaB and caspase-1 activation. ASC2 adopts a six-helix bundle structure with a prominent loop, comprising 13 amino acid residues, between helices two and three. This loop represents a divergent feature of PYDs from other domains with the DD fold. Detailed analysis of backbone 15N NMR relaxation data using both the Lipari-Szabo model-free and reduced spectral density function formalisms revealed no evidence of contiguous stretches of polypeptide chain with dramatically increased internal motion, except at the extreme N and C termini. Some mobility in the fast, picosecond to nanosecond timescale, was seen in helix 3 and the preceding alpha2-alpha3 loop, in stark contrast to the complete disorder seen in the corresponding region of the NALP1 PYD.
Background: ASC2, a PYD-only protein that functions as a modulator of multidomain PYD-containing proteins involved in NF-kappaB and caspase-1 activation. ASC2 adopts a six-helix bundle structure with a prominent loop, comprising 13 amino acid residues, between helices two and three. This loop represents a divergent feature of PYDs from other domains with the DD fold. Detailed analysis of backbone 15N NMR relaxation data using both the Lipari-Szabo model-free and reduced spectral density function formalisms revealed no evidence of contiguous stretches of polypeptide chain with dramatically increased internal motion, except at the extreme N and C termini. Some mobility in the fast, picosecond to nanosecond timescale, was seen in helix 3 and the preceding alpha2-alpha3 loop, in stark contrast to the complete disorder seen in the corresponding region of the NALP1 PYD.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Pyrin domain-containing protein 1
NCBI Official Synonym Full Names
pyrin domain containing 1
NCBI Official Symbol
PYDC1
NCBI Official Synonym Symbols
ASC2; POP1; PYC1; cPOP1
NCBI Protein Information
pyrin domain-containing protein 1
UniProt Protein Name
Pyrin domain-containing protein 1
UniProt Gene Name
PYDC1
UniProt Synonym Gene Names
cPOP1
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Product Notes
The Human PYDC1 pydc1 (Catalog #AAA331204) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331204 ELISA Kit recognizes Human PYDC1. It is sometimes possible for the material contained within the vial of "Pyrin Domain-Containing Protein 1 (PYDC1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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