Standard Curve (Sample)
(Fig.1. Human Pyrin domain-containing protein 2 (PYDC2) Standard Curve.)
Human Pyrin Domain-Containing Protein 2 (PYDC2) ELISA Kit | PYDC2 elisa kit
Human Pyrin Domain-Containing Protein 2 (PYDC2) ELISA Kit
Gene Names
PYDC2; POP2; cPOP2
Reactivity
Human
Synonyms
Pyrin Domain-Containing Protein 2 (PYDC2); N/A; Human Pyrin Domain-Containing Protein 2 (PYDC2) ELISA Kit; PYDC2; POP2; cPOP2; pyrin-only protein 2; PYDC2 elisa kit
Reactivity
Human
Specificity
Human Pyrin domain-containing protein 2 (PYDC2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PYDC2. No significant cross-reactivity or interference between Human PYDC2 and analogues was observed.
Sequence Length
97
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Pyrin domain-containing protein 2 (PYDC2) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Pyrin domain-containing protein 2 (PYDC2) Standard Curve.)
Related Product Information for PYDC2 elisa kit
Description: This Human Pyrin domain-containing protein 2 (PYDC2) ELISA Kit employs a two-site sandwich ELISA to quantitate PYDC2 in samples. An antibody specific for PYDC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PYDC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PYDC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYDC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Pyrin domain (PYD) proteins have recently emerged as important signaling molecules involved in the development of innate immunity against intracellular pathogens through activation of inflammatory mediator pathways. It is modulated by a cellular PYD-only protein (cPOP1), which binds to ASC and interferes with the recruitment of ASC to activated PYD-NLRs. POP (cPOP2), which shows highest homology to the PYD of PAN1. cPOP2 binds to ASC and PAN1, thereby blocking formation of cryopyrin and PAN1-containing inflammasomes, activation of caspase-1, and subsequent processing and secretion of bioactive interleukin-1beta. Existence of a second cPOP provides additional insights into inflammasome formation and suggests that POPs might be a common regulatory mechanism to "fine-tune" the activity of specific PYD-NLR family protein-containing inflammasomes.
Background: Pyrin domain (PYD) proteins have recently emerged as important signaling molecules involved in the development of innate immunity against intracellular pathogens through activation of inflammatory mediator pathways. It is modulated by a cellular PYD-only protein (cPOP1), which binds to ASC and interferes with the recruitment of ASC to activated PYD-NLRs. POP (cPOP2), which shows highest homology to the PYD of PAN1. cPOP2 binds to ASC and PAN1, thereby blocking formation of cryopyrin and PAN1-containing inflammasomes, activation of caspase-1, and subsequent processing and secretion of bioactive interleukin-1beta. Existence of a second cPOP provides additional insights into inflammasome formation and suggests that POPs might be a common regulatory mechanism to "fine-tune" the activity of specific PYD-NLR family protein-containing inflammasomes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
pyrin domain-containing protein 2
NCBI Official Synonym Full Names
pyrin domain containing 2
NCBI Official Symbol
PYDC2
NCBI Official Synonym Symbols
POP2; cPOP2
NCBI Protein Information
pyrin domain-containing protein 2
UniProt Protein Name
Pyrin domain-containing protein 2
UniProt Gene Name
PYDC2
UniProt Synonym Gene Names
POP2
UniProt Entry Name
PYDC2_HUMAN
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Product Notes
The Human PYDC2 pydc2 (Catalog #AAA331203) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331203 ELISA Kit recognizes Human PYDC2. It is sometimes possible for the material contained within the vial of "Pyrin Domain-Containing Protein 2 (PYDC2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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