Standard Curve (Sample)
(Fig.1. Human Pyrroline-5-carboxylate reductase 1, mitochondrial (PYCR1) Standard Curve.)
Human Pyrroline-5-Carboxylate Reductase 1, Mitochondrial (PYCR1) ELISA Kit | PYCR1 elisa kit
Human Pyrroline-5-Carboxylate Reductase 1, Mitochondrial (PYCR1) ELISA Kit
Gene Names
PYCR1; P5C; P5CR; PRO3; PYCR; PIG45; PP222; ARCL2B; ARCL3B
Reactivity
Human
Synonyms
Pyrroline-5-Carboxylate Reductase 1, Mitochondrial (PYCR1); N/A; Human Pyrroline-5-Carboxylate Reductase 1, Mitochondrial (PYCR1) ELISA Kit; PYCR1; ARCL2B; P5C; P5CR; PIG45; PP222; PRO3; PYCR; P5C reductase; proliferation-inducing protein 45; PYCR1 elisa kit
Reactivity
Human
Specificity
Human Pyrroline-5-carboxylate reductase 1, mitochondrial (PYCR1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PYCR1. No significant cross-reactivity or interference between Human PYCR1 and analogues was observed.
Sequence Length
319
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Pyrroline-5-carboxylate reductase 1, mitochondrial (PYCR1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Pyrroline-5-carboxylate reductase 1, mitochondrial (PYCR1) Standard Curve.)
Related Product Information for PYCR1 elisa kit
Description: This Human Pyrroline-5-carboxylate reductase 1, mitochondrial (PYCR1) ELISA Kit employs a two-site sandwich ELISA to quantitate PYCR1 in samples. An antibody specific for PYCR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PYCR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PYCR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYCR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PYCR1 catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternate splicing results in two transcript variants encoding different isoforms.The 1,810-bp cDNA hybridized to a 1.85-kb mRNA in samples from human cell lines and predicated a 319-amino acid, 33.4-kD protein. PYCR1 protein colocalized with various mitochondrial markers, including delta-1-pyrroline-5-carboxylate synthetase (P5CS), which also belongs to the proline metabolic pathway. Knockdown of the orthologous genes in Xenopus and zebrafish led to epidermal hypoplasia and blistering that was accompanied by a massive increase in apoptosis.
Background: PYCR1 catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternate splicing results in two transcript variants encoding different isoforms.The 1,810-bp cDNA hybridized to a 1.85-kb mRNA in samples from human cell lines and predicated a 319-amino acid, 33.4-kD protein. PYCR1 protein colocalized with various mitochondrial markers, including delta-1-pyrroline-5-carboxylate synthetase (P5CS), which also belongs to the proline metabolic pathway. Knockdown of the orthologous genes in Xenopus and zebrafish led to epidermal hypoplasia and blistering that was accompanied by a massive increase in apoptosis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
pyrroline-5-carboxylate reductase 1, mitochondrial isoform 1
NCBI Official Synonym Full Names
pyrroline-5-carboxylate reductase 1
NCBI Official Symbol
PYCR1
NCBI Official Synonym Symbols
P5C; P5CR; PRO3; PYCR; PIG45; PP222; ARCL2B; ARCL3B
NCBI Protein Information
pyrroline-5-carboxylate reductase 1, mitochondrial
UniProt Protein Name
Pyrroline-5-carboxylate reductase 1, mitochondrial
UniProt Gene Name
PYCR1
UniProt Synonym Gene Names
P5C reductase 1; P5CR 1
UniProt Entry Name
P5CR1_HUMAN
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Product Notes
The Human PYCR1 pycr1 (Catalog #AAA331207) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331207 ELISA Kit recognizes Human PYCR1. It is sometimes possible for the material contained within the vial of "Pyrroline-5-Carboxylate Reductase 1, Mitochondrial (PYCR1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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