Standard Curve (Sample)
(Fig.1. Human R-spondin-3 (RSPO3) Standard Curve.)
Human R-Spondin-3 (RSPO3) ELISA Kit | RSPO3 elisa kit
Human R-Spondin-3 (RSPO3) ELISA Kit
Gene Names
RSPO3; PWTSR; THSD2; CRISTIN1
Reactivity
Human
Synonyms
R-Spondin-3 (RSPO3); N/A; Human R-Spondin-3 (RSPO3) ELISA Kit; RSPO3; CRISTIN1; FLJ14440; PWTSR; THSD2; R-spondin 3; thrombospondin; type I; domain containing 2; thrombospondin-like; RSPO3 elisa kit
Reactivity
Human
Specificity
Human R-spondin-3 (RSPO3) ELISA Kit has high sensitivity and excellent specificity for detection of Human RSPO3. No significant cross-reactivity or interference between Human RSPO3 and analogues was observed.
Sequence Length
272
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human R-spondin-3 (RSPO3) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human R-spondin-3 (RSPO3) Standard Curve.)
Related Product Information for RSPO3 elisa kit
Description: This Human R-spondin-3 (RSPO3) ELISA Kit employs a two-site sandwich ELISA to quantitate RSPO3 in samples. An antibody specific for RSPO3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RSPO3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RSPO3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RSPO3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: RSPO3 encodes a member of the thrombospondin type 1 repeat supergene family. In addition, the protein contains a furin-like cysteine-rich region. Furin-like repeat domains have been found in a variety of eukaryotic proteins involved in the mechanism of signal transduction by receptor tyrosine kinases.The 272-amino acid protein has an N-terminal cys-rich domain (CRD) and a central thrombospondin type-1 (THBS1) repeat (TSR) domain. Northern blot analysis detected 3.0- and 4.0-kb transcripts in placenta, lung, and skeletal muscle. RT-PCR revealed ubiquitous expression, with highest levels in placenta, small intestine, fetal thymus, and lymph node. RSPO3 expression was detected in all tumor tissues examined except a lung carcinoma and an ovarian carcinoma.
Background: RSPO3 encodes a member of the thrombospondin type 1 repeat supergene family. In addition, the protein contains a furin-like cysteine-rich region. Furin-like repeat domains have been found in a variety of eukaryotic proteins involved in the mechanism of signal transduction by receptor tyrosine kinases.The 272-amino acid protein has an N-terminal cys-rich domain (CRD) and a central thrombospondin type-1 (THBS1) repeat (TSR) domain. Northern blot analysis detected 3.0- and 4.0-kb transcripts in placenta, lung, and skeletal muscle. RT-PCR revealed ubiquitous expression, with highest levels in placenta, small intestine, fetal thymus, and lymph node. RSPO3 expression was detected in all tumor tissues examined except a lung carcinoma and an ovarian carcinoma.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
R-spondin-3
NCBI Official Synonym Full Names
R-spondin 3
NCBI Official Symbol
RSPO3
NCBI Official Synonym Symbols
PWTSR; THSD2; CRISTIN1
NCBI Protein Information
R-spondin-3
UniProt Protein Name
R-spondin-3
UniProt Gene Name
RSPO3
UniProt Synonym Gene Names
PWTSR; THSD2; hPWTSR; hRspo3
UniProt Entry Name
RSPO3_HUMAN
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Product Notes
The Human RSPO3 rspo3 (Catalog #AAA330981) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330981 ELISA Kit recognizes Human RSPO3. It is sometimes possible for the material contained within the vial of "R-Spondin-3 (RSPO3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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