Standard Curve (Sample)
(Fig.1. Human RAC-beta serine/threonine-protein kinase (AKT2) Standard Curve.)
Human RAC-beta Serine/Threonine-Protein Kinase (AKT2) ELISA Kit | AKT2 elisa kit
Human RAC-beta Serine/Threonine-Protein Kinase (AKT2) ELISA Kit
Gene Names
AKT2; PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
Reactivity
Human
Synonyms
RAC-beta Serine/Threonine-Protein Kinase (AKT2); N/A; Human RAC-beta Serine/Threonine-Protein Kinase (AKT2) ELISA Kit; AKT2; PKBB; PKBBETA; PRKBB; RAC-BETA; AKT2 kinase; Murine thymoma viral (v-akt) homolog-2; rac protein kinase beta; AKT2 elisa kit
Reactivity
Human
Specificity
Human RAC-beta serine/threonine-protein kinase (AKT2) ELISA Kit has high sensitivity and excellent specificity for detection of Human AKT2. No significant cross-reactivity or interference between Human AKT2 and analogues was observed.
Sequence Length
481
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human RAC-beta serine/threonine-protein kinase (AKT2) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human RAC-beta serine/threonine-protein kinase (AKT2) Standard Curve.)
Related Product Information for AKT2 elisa kit
Description: This Human RAC-beta serine/threonine-protein kinase (AKT2) ELISA Kit employs a two-site sandwich ELISA to quantitate AKT2 in samples. An antibody specific for AKT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AKT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AKT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AKT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phosphorylating several known proteins. Mice lacking Akt2 have a normal body mass, but display a profound diabetic phenotype, indicating that Akt2 plays a key role in signal transduction downstream of the insulin receptor. Mice lacking Akt2 show worse outcome in breast cancer initaited by the large T antigen as well as the neu oncogene.
Background: AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phosphorylating several known proteins. Mice lacking Akt2 have a normal body mass, but display a profound diabetic phenotype, indicating that Akt2 plays a key role in signal transduction downstream of the insulin receptor. Mice lacking Akt2 show worse outcome in breast cancer initaited by the large T antigen as well as the neu oncogene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
RAC-beta serine/threonine-protein kinase isoform 1
NCBI Official Synonym Full Names
AKT serine/threonine kinase 2
NCBI Official Symbol
AKT2
NCBI Official Synonym Symbols
PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
NCBI Protein Information
RAC-beta serine/threonine-protein kinase
UniProt Protein Name
RAC-beta serine/threonine-protein kinase
UniProt Gene Name
AKT2
UniProt Synonym Gene Names
PKB beta
UniProt Entry Name
AKT2_HUMAN
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Product Notes
The Human AKT2 akt2 (Catalog #AAA330852) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330852 ELISA Kit recognizes Human AKT2. It is sometimes possible for the material contained within the vial of "RAC-beta Serine/Threonine-Protein Kinase (AKT2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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