Standard Curve (Sample)
(Fig.1. Human Reticulon-4 (RTN4) Standard Curve.)
Human Reticulon-4 (RTN4) ELISA Kit | RTN4 elisa kit
Human Reticulon-4 (RTN4) ELISA Kit
Gene Names
RTN4; ASY; NSP; NOGO; RTN-X; NSP-CL; RTN4-A; RTN4-C; RTN4-B1; RTN4-B2; NI220/250; Nbla00271; Nbla10545
Reactivity
Human
Synonyms
Reticulon-4 (RTN4); N/A; Human Reticulon-4 (RTN4) ELISA Kit; RTN4; ASY; NI220/250; NOGO; NOGO-A; NOGOC; NSP; NSP-CL; Nbla00271; Nbla10545; Nogo-B; Nogo-C; RTN-X; RTN4-A; RTN4-B1; RTN4-B2; RTN4-C; Human NogoA; My043 protein; OTTHUMP00000201714; OTTHUMP00000201801; OTTHU; RTN4 elisa kit
Reactivity
Human
Specificity
Human Reticulon-4 (RTN4) ELISA Kit has high sensitivity and excellent specificity for detection of Human RTN4. No significant cross-reactivity or interference between Human RTN4 and analogues was observed.
Sequence Length
1192
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Reticulon-4 (RTN4) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Reticulon-4 (RTN4) Standard Curve.)
Related Product Information for RTN4 elisa kit
Description: This Human Reticulon-4 (RTN4) ELISA Kit employs a two-site sandwich ELISA to quantitate RTN4 in samples. An antibody specific for RTN4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RTN4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RTN4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RTN4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Reticulon-4 is a protein belongs to the family of reticulon-encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified. Isoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 is testis-specific.
Background: Reticulon-4 is a protein belongs to the family of reticulon-encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified. Isoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 is testis-specific.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Reticulon-4
NCBI Official Synonym Full Names
reticulon 4
NCBI Official Symbol
RTN4
NCBI Official Synonym Symbols
ASY; NSP; NOGO; RTN-X; NSP-CL; RTN4-A; RTN4-C; RTN4-B1; RTN4-B2; NI220/250; Nbla00271; Nbla10545
NCBI Protein Information
reticulon-4
UniProt Protein Name
Reticulon-4
UniProt Gene Name
RTN4
UniProt Synonym Gene Names
KIAA0886; NOGO; Nogo protein; NSP
UniProt Entry Name
RTN4_HUMAN
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Product Notes
The Human RTN4 rtn4 (Catalog #AAA330979) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330979 ELISA Kit recognizes Human RTN4. It is sometimes possible for the material contained within the vial of "Reticulon-4 (RTN4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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