Human Retinol binding protein 4 (RBP4) ELISA Kit | RBP4 elisa kit

Retinol binding protein 4 (RBP4): Human, (ELISA)

Cat. Num. AAA60541

• Gene Names: RBP4A; RBP; RBP4
• Reactivity: Human
• Synonyms: Retinol binding protein 4 (RBP4); N/A; Retinol binding protein 4 (RBP4): Human, (ELISA); Human retinol binding protein (RBP4); RBP4 elisa kit

• Reactivity: Human
• Specificity: The antibody pair used in this assay is specific to human RBP4 and does not crossreact with mouse and rat RBP4, and other cytokine or hormone molecules.
• Sequence Length: 196
• Assay Type: Quantitative Sandwich
• Samples: Cell culture supernates, serum or plasma
• Sensitivity: 3.12 ng/mL

• Intra-assay Precision: Three samples of known concentration were tested 16 times on one plate.
• Inter-assay Precision: Three samples of known concentration were tested in 10 separate assays.

Standard Curve (Sample)

Related Product Information for RBP4 elisa kit

Background: Retinol binding protein 4 (RBP4), originally known as a specific transport of retinol in blood, is also a novel inflammatory and insulin resistance marker1.

Principle of the Assay: This assay is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies against human RBP4. The microtiter plate is pre-coated with a monoclonal antibody specific for human RBP4 and the nonspecific binding sites are blocked. Standards and samples are pipetted into the wells and any human RBP4 present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked monoclonal antibody specific for human RBP4 is added to the wells. After wash step to remove any unbound reagents, an HRP substrate solution is added and color develops in proportion to the amount of human RBP4 bound initially. The assay is stopped, and the optical density of the wells is determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human RBP4, the unknown sample concentration can be interpolated from a reference curve included in each assay.

Intended Uses: This Human RBP4 ELISA kit is designed for quantification of human RBP4 in serum, plasma, and adipocyte extracts or cell culture supernate samples.

References

1.The metabolic role of retinol binding protein 4: an update. Christou GA, Tselepis AD, Kiortsis DN. Horm Metab Res. 2012 44(1):6-14.
2. RBP4: a controversial adipokine. Kotnik P, Fischer-Posovszky P, Wabitsch M. Eur J Endocrinol. 2011 Nov;165(5):703-11.

NCBI and Uniprot Product Information

• NCBI GI #: 45382541
• NCBI GeneID: 396166
• NCBI GenBank Nucleotide #: NP_990569.1
• UniProt Accession #: P41263
• NCBI Official Full Name: retinol-binding protein 4
• NCBI Official Synonym Full Names: retinol binding protein 4 A, plasma
• NCBI Official Symbol: RBP4A
• NCBI Official Synonym Symbols: RBP; RBP4
• NCBI Protein Information: retinol-binding protein 4
• UniProt Protein Name: Retinol-binding protein 4
• UniProt Gene Name: RBP4
• UniProt Synonym Gene Names: PRBP; RBP
• UniProt Entry Name: RET4_CHICK