Human Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2) ELISA Kit | Rock2 elisa kit

Human Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2) ELISA Kit

Cat. Num. AAA136702

• Gene Names: ROCK2; ROCK-II
• Reactivity: Human
• Synonyms: Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2); N/A; Human Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2) ELISA Kit; Rock2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rock2. No significant cross-reactivity or interference between Rock2 and analogues was observed.
• Sequence Length: 1388
• Assay Type: Quantitative Sandwich
• Samples: Human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.061ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rock2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rock2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for Rock2 elisa kit

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to Rock2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Rock2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rock2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rock2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Rock2 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Product Categories/Family for Rock2 elisa kit

Signal transduction; Enzyme & Kinase; Tumor immunity; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 41872583
• NCBI GeneID: 9475
• NCBI Accession #: NP_004841.2
• NCBI GenBank Nucleotide #: NM_004850.3
• UniProt Accession #: O75116; Q53QZ0; Q53SJ7; Q9UQN5
• Molecular Weight: 160,900 Da
• NCBI Official Full Name: rho-associated protein kinase 2
• NCBI Official Synonym Full Names: Rho-associated, coiled-coil containing protein kinase 2
• NCBI Official Symbol: ROCK2
• NCBI Official Synonym Symbols: ROCK-II
• NCBI Protein Information: rho-associated protein kinase 2
• UniProt Protein Name: Rho-associated protein kinase 2
• UniProt Gene Name: ROCK2
• UniProt Synonym Gene Names: KIAA0619; ROCK-II
• UniProt Entry Name: ROCK2_HUMAN