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product-image-AAA80786_SC15.png Standard Curve (Sample)

Human Rho-associated, coiled-coil containing protein kinase 2 ELISA Kit | ROCK2 elisa kit

Human Rho-associated coiled-coil containing protein kinase 2, Rock-2 ELISA Kit

Gene Names
ROCK2; ROCK-II
Reactivity
Human
Synonyms
Rho-associated, coiled-coil containing protein kinase 2; N/A; Human Rho-associated coiled-coil containing protein kinase 2, Rock-2 ELISA Kit; Human Rho-associated coiled-coil containing protein kinase 2 (Rock-2) ELISA Kit; KIAA0619; ; Rho-associated; coiled-coil containing protein kinase 2; ROCK2 elisa kit
Ordering
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human ROCK2. No significant cross-reactivity or interference between Human ROCK2 and analogues was observed.
Sequence Length
1388
Assay Type
Sandwich
Detection Range
0.625 ng/ml-40 ng/ml
Sensitivity
0.156ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

product-image-AAA80786_SC15.png Standard Curve (Sample)
Related Product Information for ROCK2 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Rock-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Rock-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Rock-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Rock-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
160,900 Da
NCBI Official Full Name
rho-associated protein kinase 2
NCBI Official Synonym Full Names
Rho-associated, coiled-coil containing protein kinase 2
NCBI Official Symbol
ROCK2
NCBI Official Synonym Symbols
ROCK-II
NCBI Protein Information
rho-associated protein kinase 2; p164 ROCK-2; rho-associated, coiled-coil-containing protein kinase II
UniProt Protein Name
Rho-associated protein kinase 2
UniProt Gene Name
ROCK2
UniProt Synonym Gene Names
KIAA0619; ROCK-II
UniProt Entry Name
ROCK2_HUMAN

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Product Notes

The Human ROCK2 rock2 (Catalog #AAA80786) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA80786 ELISA Kit recognizes Human ROCK2. It is sometimes possible for the material contained within the vial of "Rho-associated, coiled-coil containing protein kinase 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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