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product-image-AAA246783_SC15.jpg Standard Curve (Sample)

Human Rho related GTP binding protein RhoC (RHOC) ELISA Kit | RHOC elisa kit

Human Rho related GTP binding protein RhoC (RHOC) ELISA Kit

Gene Names
RHOC; H9; ARH9; ARHC; RHOH9
Reactivity
Human
Synonyms
Rho related GTP binding protein RhoC (RHOC); N/A; Human Rho related GTP binding protein RhoC (RHOC) ELISA Kit; RHOC elisa kit
Ordering
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of RHOC. No significant cross-reactivity or interference between RHOC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RHOC and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.

Standard Curve (Sample)

product-image-AAA246783_SC15.jpg Standard Curve (Sample)
Related Product Information for RHOC elisa kit
Principle of the Assay: RHOC ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-RHOC antibody and an RHOC-HRP conjugate. The assay sample and buffer are incubated together with RHOC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RHOC concentration since RHOC from samples and RHOC-HRP conjugate compete for the anti-RHOC antibody binding site. Since the number of sites is limited, as more sites are occupied by RHOC from the sample, fewer sites are left to bind RHOC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RHOC concentration in each sample is interpolated from this standard curve.

Intended Uses: This RHOC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human RHOC. This ELISA kit for research use only, not for therapeutic or test applications!
Product Categories/Family for RHOC elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
389
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,006 Da
NCBI Official Full Name
rho-related GTP-binding protein RhoC
NCBI Official Synonym Full Names
ras homolog family member C
NCBI Official Symbol
RHOC
NCBI Official Synonym Symbols
H9; ARH9; ARHC; RHOH9
NCBI Protein Information
rho-related GTP-binding protein RhoC; rhoC GTPase; oncogene RHO H9; rho cDNA clone 9; RAS-related homolog 9; small GTP binding protein RhoC; ras homolog gene family, member C
UniProt Protein Name
Rho-related GTP-binding protein RhoC
UniProt Gene Name
RHOC
UniProt Synonym Gene Names
ARH9; ARHC; h9
UniProt Entry Name
RHOC_HUMAN

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Product Notes

The Human RHOC rhoc (Catalog #AAA246783) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA246783 ELISA Kit recognizes Human RHOC. It is sometimes possible for the material contained within the vial of "Rho related GTP binding protein RhoC (RHOC), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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