Standard Curve (Sample)
(Fig.1. Human Ribonucleoside-diphosphate reductase large subunit (RRM1) Standard Curve.)
Human Ribonucleoside-Diphosphate Reductase Large Subunit (RRM1) ELISA Kit | RRM1 elisa kit
Human Ribonucleoside-Diphosphate Reductase Large Subunit (RRM1) ELISA Kit
Gene Names
RRM1; R1; RR1; RIR1
Reactivity
Human
Synonyms
Ribonucleoside-Diphosphate Reductase Large Subunit (RRM1); N/A; Human Ribonucleoside-Diphosphate Reductase Large Subunit (RRM1) ELISA Kit; RRM1; R1; RIR1; RR1; ribonucleoside-diphosphate reductase M1 chain; ribonucleotide reductase M1 polypeptide; ribonucleotide reductase; R1 subunit; large subunit; RRM1 elisa kit
Reactivity
Human
Specificity
Human Ribonucleoside-diphosphate reductase large subunit (RRM1) ELISA Kit has high sensitivity and excellent specificity for detection of Human RRM1. No significant cross-reactivity or interference between Human RRM1 and analogues was observed.
Sequence Length
792
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Ribonucleoside-diphosphate reductase large subunit (RRM1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Ribonucleoside-diphosphate reductase large subunit (RRM1) Standard Curve.)
Related Product Information for RRM1 elisa kit
Description: This Human Ribonucleoside-diphosphate reductase large subunit (RRM1) ELISA Kit employs a two-site sandwich ELISA to quantitate RRM1 in samples. An antibody specific for RRM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RRM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RRM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RRM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: RRM1 encodes one of two non-identical subunits which constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region. This gene is oriented in a head-to-tail configuration with the stromal interaction molecule 1 gene (STIM1), with the 3' end of STIM1 situated 1.6 kb from the 5' end of this gene.
Background: RRM1 encodes one of two non-identical subunits which constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region. This gene is oriented in a head-to-tail configuration with the stromal interaction molecule 1 gene (STIM1), with the 3' end of STIM1 situated 1.6 kb from the 5' end of this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Ribonucleoside-diphosphate reductase large subunit
NCBI Official Synonym Full Names
ribonucleotide reductase catalytic subunit M1
NCBI Official Symbol
RRM1
NCBI Official Synonym Symbols
R1; RR1; RIR1
NCBI Protein Information
ribonucleoside-diphosphate reductase large subunit
UniProt Protein Name
Ribonucleoside-diphosphate reductase large subunit
UniProt Gene Name
RRM1
UniProt Synonym Gene Names
RR1
UniProt Entry Name
RIR1_HUMAN
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Product Notes
The Human RRM1 rrm1 (Catalog #AAA330986) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330986 ELISA Kit recognizes Human RRM1. It is sometimes possible for the material contained within the vial of "Ribonucleoside-Diphosphate Reductase Large Subunit (RRM1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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