Standard Curve (Sample)
(Fig.1. Human Ryanodine receptor 1 (RYR1) Standard Curve.)
Human Ryanodine Receptor 1 (RYR1) ELISA Kit | RYR1 elisa kit
Human Ryanodine Receptor 1 (RYR1) ELISA Kit
Gene Names
RYR1; CCO; MHS; RYR; MHS1; RYDR; SKRR; RYR-1; PPP1R137
Reactivity
Human
Synonyms
Ryanodine Receptor 1 (RYR1); N/A; Human Ryanodine Receptor 1 (RYR1) ELISA Kit; RYR1; CCO; MHS; MHS1; RYDR; RYR; SKRR; central core disease of muscle; ryanodine receptor type1; sarcoplasmic reticulum calcium release channel; skeletal muscle ryanodine receptor; type 1-like ryanodine recep; RYR1 elisa kit
Reactivity
Human
Specificity
Human Ryanodine receptor 1 (RYR1) ELISA Kit has high sensitivity and excellent specificity for detection of Human RYR1. No significant cross-reactivity or interference between Human RYR1 and analogues was observed.
Sequence Length
5038
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Ryanodine receptor 1 (RYR1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Ryanodine receptor 1 (RYR1) Standard Curve.)
Related Product Information for RYR1 elisa kit
Description: This Human Ryanodine receptor 1 (RYR1) ELISA Kit employs a two-site sandwich ELISA to quantitate RYR1 in samples. An antibody specific for RYR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RYR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RYR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RYR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Ryanodine receptor 1 is a ryanodine receptor found in skeletal muscle. The encoded protein functions as a calcium release channel in the sarcoplasmic reticulum but also serves to connect the sarcoplasmic reticulum and transverse tubule. Mutations in this gene are associated with malignant hyperthermia susceptibility, central core disease, and minicore myopathy with external ophthalmoplegia. Alternatively spliced transcripts encoding different isoforms have been described. Communication between transverse-tubules and sarcoplasmic reticulum. Contraction of skeletal muscle is triggered by release of calcium ions from SR following depolarization of T-tubules.Skeletal muscle and brain (cerebellum and hippocampus).
Background: Ryanodine receptor 1 is a ryanodine receptor found in skeletal muscle. The encoded protein functions as a calcium release channel in the sarcoplasmic reticulum but also serves to connect the sarcoplasmic reticulum and transverse tubule. Mutations in this gene are associated with malignant hyperthermia susceptibility, central core disease, and minicore myopathy with external ophthalmoplegia. Alternatively spliced transcripts encoding different isoforms have been described. Communication between transverse-tubules and sarcoplasmic reticulum. Contraction of skeletal muscle is triggered by release of calcium ions from SR following depolarization of T-tubules.Skeletal muscle and brain (cerebellum and hippocampus).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Ryanodine receptor 1
NCBI Official Synonym Full Names
ryanodine receptor 1
NCBI Official Symbol
RYR1
NCBI Official Synonym Symbols
CCO; MHS; RYR; MHS1; RYDR; SKRR; RYR-1; PPP1R137
NCBI Protein Information
ryanodine receptor 1
UniProt Protein Name
Ryanodine receptor 1
UniProt Gene Name
RYR1
UniProt Synonym Gene Names
RYDR; RYR-1; RyR1
UniProt Entry Name
RYR1_HUMAN
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Product Notes
The Human RYR1 ryr1 (Catalog #AAA330974) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330974 ELISA Kit recognizes Human RYR1. It is sometimes possible for the material contained within the vial of "Ryanodine Receptor 1 (RYR1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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