Human S100A12 PicoKine ELISA Kit | S100A12 elisa kit

Human S100A12 PicoKine ELISA Kit

Cat. Num. AAA46144

• Gene Names: S100A12; p6; CAGC; CGRP; MRP6; CAAF1; MRP-6; ENRAGE
• Reactivity: Human
• Synonyms: S100A12 PicoKine; N/A; Human S100A12 PicoKine ELISA Kit; Protein S100-A12; CAAF1; CAGC; Calcitermin; Calcium binding protein in amniotic fluid; Calcium-binding protein in amniotic fluid 1; Calgranulin C; Calgranulin related protein; Calgranulin-C; Calgranulin-related protein; CGRP; EN RAGE; EN-RAGE; ENRAGE; Extracellular newly identified RAGE-binding protein; migration inhibitory factor-related protein 6; MRP6; Neutrophil S100 protein; p6; Protein S100 A12; S100 calcium binding protein A12; S100 calcium-binding protein A12 (calgranulin C); S100 calcium-binding protein A12; S100A12; S10AC_HUMAN ; S100A12 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human S100A12
• Sequence Length: 92
• Assay Range: 312pg/ml-20,000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of human S100A12 in cell culture supernates, serum and plasma(heparin, EDTA).

• Immunogen: E Coli, M1-E92
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 20,000pg/ml, 10,000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml human S100A12 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human S100A12 standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(ng/ml)|0.49|1.66|8.37|0.41|1.89|7.14; Standard deviation|0.002|0.097|0.451|0.022|0.113|0.414; CV(%)|5.1|5.9|5.4|5.4|6.0|5.8
• Sample Data: Concentration(pg/ml)|0|312|625|1250|2500|5000|10000|20000; O.D.|0.022|0.094|0.161|0.336|0.68|1.267|1.780|2.112
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Standard Curve (Sample)

(Human S100A12 PicoKine ELISA Kit standard curve)

Related Product Information for S100A12 elisa kit

Principle of the assay: Human S100A12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for S100A12 has been precoated onto 96-well plates. Standards(E Coli,M1-E92) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for S100A12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human S100A12 amount of sample captured in plate.

Background: S100 calcium-binding protein A12 (S100A12) is a protein that in humans is encoded by the S100A12 gene. It is also known as calgranulin C. By direct R-banding FISH, the S100A12 gene is mapped to chromosome 1q21.2-q22. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. And this protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities.

NCBI and Uniprot Product Information

• NCBI GI #: 5032059
• NCBI GeneID: 6283
• NCBI Accession #: NP_005612.1
• NCBI GenBank Nucleotide #: NM_005621.1
• UniProt Accession #: P80511; P83219; Q5SY66; Q7M4R1
• Molecular Weight: 10,575 Da
• NCBI Official Full Name: protein S100-A12
• NCBI Official Synonym Full Names: S100 calcium binding protein A12
• NCBI Official Symbol: S100A12
• NCBI Official Synonym Symbols: p6; CAGC; CGRP; MRP6; CAAF1; MRP-6; ENRAGE
• NCBI Protein Information: protein S100-A12
• UniProt Protein Name: Protein S100-A12
• UniProt Gene Name: S100A12
• UniProt Synonym Gene Names: CAAF1; CAGC; EN-RAGE; MRP-6; p6
• UniProt Entry Name: S10AC_HUMAN