Standard Curve (Sample)
Human Secreted phosphoprotein 24 ELISA Kit | SPP2 elisa kit
Human Secreted phosphoprotein 24 ELISA Kit
Gene Names
SPP2; SPP24; SPP-24
Reactivity
Human
Synonyms
Secreted phosphoprotein 24; N/A; Human Secreted phosphoprotein 24 ELISA Kit; SPP2 elisa kit
Reactivity
Human
Samples
Human Serum
Sensitivity
1.0 U/ml.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SPP2 elisa kit
Intended Uses: This Sphingomyelin IgG/M ELISA is a solid phase enzyme immunoassay with highly purified sphingomyelin and native human beta2-glycoprotein I for the quantitative and qualitative detection of IgG and/or IgM antibodies against sphingomyelin in human serum. These antibodies recognize specific epitopes on a complex composed out of sphingomyelin and beta2-glycoprotein I. For research use only.
Principle of the Assay: Serum samples diluted 1:101 are incubated in the microplates coated with the specific antigen. Antibodies, if present in the specimen, bind to the antigen. The unbound fraction is washed off in the following step. Afterwards anti-human immunoglobulins conjugated to horseradish peroxidase (conjugate) are incubated and react with the antigen-antibody complex of the samples in the microplates. Unbound conjugate is washed off in the following step. Addition of TMB-substrate generates an enzymatic colorimetric (blue) reaction, which is stopped by diluted acid (color changes to yellow). The intensity of color formation from the chromogen is a function of the amount of conjugate bound to the antigen-antibody complex and this is proportional to the initial concentration of the respective antibodies in the sample!!Background/Introduction: Antibodies against sphingomyelin belong to the group of anti-phospholipid antibodies such as cardiolipin, phosphatidyl-serine,-inositol,-choline and phosphatidic acid. Phospholipids are components of biological membranes.
Principle of the Assay: Serum samples diluted 1:101 are incubated in the microplates coated with the specific antigen. Antibodies, if present in the specimen, bind to the antigen. The unbound fraction is washed off in the following step. Afterwards anti-human immunoglobulins conjugated to horseradish peroxidase (conjugate) are incubated and react with the antigen-antibody complex of the samples in the microplates. Unbound conjugate is washed off in the following step. Addition of TMB-substrate generates an enzymatic colorimetric (blue) reaction, which is stopped by diluted acid (color changes to yellow). The intensity of color formation from the chromogen is a function of the amount of conjugate bound to the antigen-antibody complex and this is proportional to the initial concentration of the respective antibodies in the sample!!Background/Introduction: Antibodies against sphingomyelin belong to the group of anti-phospholipid antibodies such as cardiolipin, phosphatidyl-serine,-inositol,-choline and phosphatidic acid. Phospholipids are components of biological membranes.
Product Categories/Family for SPP2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,338 Da
NCBI Official Full Name
secreted phosphoprotein 24
NCBI Official Synonym Full Names
secreted phosphoprotein 2, 24kDa
NCBI Official Symbol
SPP2
NCBI Official Synonym Symbols
SPP24; SPP-24
NCBI Protein Information
secreted phosphoprotein 24; secreted phosphoprotein 2, 24kD
UniProt Protein Name
Secreted phosphoprotein 24
UniProt Gene Name
SPP2
UniProt Synonym Gene Names
SPP24; Spp-24
UniProt Entry Name
SPP24_HUMAN
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Product Notes
The Human SPP2 spp2 (Catalog #AAA244522) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA244522 ELISA Kit recognizes Human SPP2. It is sometimes possible for the material contained within the vial of "Secreted phosphoprotein 24, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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