Human Semaphorin 4D (SEMA4D) ELISA Kit | SEMA4D elisa kit

Human Semaphorin 4D (SEMA4D) ELISA Kit

Cat. Num. AAA20641

• Gene Names: SEMA4D; CD100; SEMAJ; coll-4; C9orf164; M-sema-G
• Reactivity: Human
• Synonyms: Semaphorin 4D (SEMA4D); N/A; Human Semaphorin 4D (SEMA4D) ELISA Kit; CD100; A8; BB18; GR3; M-Sema G; M-Sema-G; SEMAJ; Coll-4; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 4D; SEMA4D elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SEMA4D. No significant cross-reactivity or interference between SEMA4D and analogues was observed.
• Sequence Length: 862
• Assay Type: Quantitative Sandwich
• Samples: Human serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Detection Range: 31.2-2,000pg/mL
• Sensitivity: < 12.1pg/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SEMA4D were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SEMA4D were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for SEMA4D elisa kit

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to SEMA4D. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to SEMA4D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SEMA4D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SEMA4D in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SEMA4D in human serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Product Categories/Family for SEMA4D elisa kit

CD & Adhesion molecule; Tumor immunity; Infection immunity; Immune molecule

References

Semaphorin 4D levels in heart failure patients: a potential novel biomarker of acute heart failure?; ESC Heart Fail; 2018 Aug;5(4):603-609.; PMID: 29524314; Nadav Willner; Department of Internal Medicine B, Bnai Zion Medical Center, Haifa, Israel.

NCBI and Uniprot Product Information

• NCBI GI #: 214010218
• NCBI GeneID: 10507
• NCBI Accession #: NP_006369.3
• NCBI GenBank Nucleotide #: NM_006378.3
• Molecular Weight: 82,164 Da
• NCBI Official Full Name: semaphorin-4D isoform 1
• NCBI Official Synonym Full Names: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
• NCBI Official Symbol: SEMA4D
• NCBI Official Synonym Symbols: CD100; SEMAJ; coll-4; C9orf164; M-sema-G
• NCBI Protein Information: semaphorin-4D
• UniProt Protein Name: Semaphorin-4D
• UniProt Gene Name: SEMA4D
• UniProt Synonym Gene Names: C9orf164; CD100; SEMAJ
• UniProt Entry Name: SEM4D_HUMAN