Standard Curve (Sample)
Human Semaphorin-4D ELISA Kit | SEMA4D elisa kit
Human Semaphorin-4D ELISA Kit
Gene Names
SEMA4D; CD100; SEMAJ; coll-4; C9orf164; M-sema-G
Reactivity
Human
Synonyms
Semaphorin-4D; N/A; Human Semaphorin-4D ELISA Kit; SEMA4D/GR3/A8/BB18/CD100/COLL-4/M-sema-G/SEMA4D/SEMAJ/A8/CD100 antigen/CD100M-sema-G/coll-4/immunoglobulin domain (Ig); transmembrane domain (TM) and shortcytoplasmic domain; 4D/sema domain; immunoglobulin domain (Ig); (semaphorin) 4D/semaphorin-4D; SEMA4D elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of SEMA4D. No significant cross-reactivity or interference between SEMA4D and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the crossreactivity detection between SEMA4D and all the analogues, therefore, cross reaction may still exist.
Sequence Length
862
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
125-8000pg/ml
Sensitivity
75pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SEMA4D were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SEMA4D were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SEMA4D elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- SEMA4D antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- SEMA4D antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the SEMA4D amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of SEMA4D can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
82,164 Da
NCBI Official Full Name
semaphorin-4D isoform 1
NCBI Official Synonym Full Names
semaphorin 4D
NCBI Official Symbol
SEMA4D
NCBI Official Synonym Symbols
CD100; SEMAJ; coll-4; C9orf164; M-sema-G
NCBI Protein Information
semaphorin-4D
UniProt Protein Name
Semaphorin-4D
UniProt Gene Name
SEMA4D
UniProt Synonym Gene Names
C9orf164; CD100; SEMAJ
UniProt Entry Name
SEM4D_HUMAN
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Product Notes
The Human SEMA4D sema4d (Catalog #AAA100504) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA100504 ELISA Kit recognizes Human SEMA4D. It is sometimes possible for the material contained within the vial of "Semaphorin-4D, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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