Standard Curve (Sample)
Human Seprase/FAP ELISA Kit | FAP elisa kit
Human Seprase/FAP PicoKine ELISA Kit
Gene Names
FAP; FAPA; DPPIV
Reactivity
Human
Synonyms
Seprase/FAP; N/A; Human Seprase/FAP PicoKine ELISA Kit; Seprase; fibroblast activation protein, alpha; 170 kDa melanoma membrane bound gelatinase; 170 kDa melanoma membrane-bound gelatinase; DPPIV; FAP; FAPA; Fibroblast activation protein alpha; Integral membrane serine protease; SEPR_HUMAN; FAP elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human FAP
Sequence Length
760
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
62.5 pg/ml - 4,000 pg/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for FAP elisa kit
Background: FAP (Fibroblast Activation Protein, Alpha) also known as FAPA or SEPRASE, is an inducible cell surface glycoprotein that was originally identified in cultured fibroblasts using monoclonal antibody F19. The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. The FAP gene is mapped on 2q24.2. FAP is most closely related to DPPIV and they share about 50% of their amino acids. FAP is catalytically active as a 170kD dimer and has dipeptidase and gelatinase activity. Its gelatinase activity requires a glycine in P2 position.FAPalpha shows 48% amino acid identity with dipeptidyl peptidase IV and 30% identity with DPP4-related protein. Northern blot analysis detected a 2.8-kb FAP-alpha mRNA in fibroblasts. Depletion of FAP-expressing cells, which made up only 2% of all tumor cells in established Lewis lung carcinomas, caused rapid hypoxic necrosis of both cancer and stromal cells in immunogenic tumors by a process involving interferon-gamma and tumor necrosis factor-alpha.
Principle of the Assay: The Picokine™ Human FAP Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human FAP with a 96-well strip plate that is pre-coated with antibody specific for FAP. The detection antibody is a biotinylated antibody specific for FAP. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit includes Human FAP protein as standards. To measure Human FAP, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human FAP in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human FAP in the sample.
Principle of the Assay: The Picokine™ Human FAP Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human FAP with a 96-well strip plate that is pre-coated with antibody specific for FAP. The detection antibody is a biotinylated antibody specific for FAP. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit includes Human FAP protein as standards. To measure Human FAP, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human FAP in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human FAP in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
26,954 Da
NCBI Official Full Name
Seprase
NCBI Official Synonym Full Names
fibroblast activation protein, alpha
NCBI Official Symbol
FAP
NCBI Official Synonym Symbols
FAPA; DPPIV
NCBI Protein Information
seprase; integral membrane serine protease; 170 kDa melanoma membrane-bound gelatinase
UniProt Protein Name
Prolyl endopeptidase FAP
UniProt Gene Name
FAP
UniProt Entry Name
SEPR_HUMAN
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Product Notes
The Human FAP fap (Catalog #AAA45896) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45896 ELISA Kit recognizes Human FAP. It is sometimes possible for the material contained within the vial of "Seprase/FAP, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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