Human SHBG PicoKine ELISA Kit | SHBG elisa kit

Human SHBG PicoKine ELISA Kit

Cat. Num. AAA46181

• Gene Names: SHBG; ABP; SBP; TEBG
• Reactivity: Human
• Synonyms: SHBG PicoKine; N/A; Human SHBG PicoKine ELISA Kit; Sex hormone-binding globulin; ABP; Androgen binding protein; SBP; Sex steroid binding protein; Sex steroid-binding protein; SHBG; SHBG_ HUMAN; TeBG; Testis specific androgen binding protein; Testis-specific androgen-binding protein; Testosterone binding beta globulin; Testosterone estradiol binding globulin; Testosterone estrogen binding globulin; Testosterone-estradiol-binding globulin; Testosterone-estrogen-binding globulin; sex hormone-binding globulin; SHBG elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human SHBG
• Sequence Length: 402
• Assay Range: 0.031nmol/L-2nmol/L
• Sensitivity: <0.001nmol/L
• Sample: For quantitative detection of human SHBG in cell culture supernates, serum and plasma(heparin, EDTA).

• Immunogen: NSO,L30-H402
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 2nmol/L, 1nmol/L, 0.5nmol/L, 0.25nmol/L, 0.125nmol/L, 0.062nmol/L, 0.031nmol/L human SHBG standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. It is recommended that each human SHBG standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(nmol/L)|0.19|0.76|1.4|0.24|0.85|1.9; Standard deviation|0.009|0.043|0.095|0.014|0.051|0.137; CV(%)|4.5|5.6|6.8|5.7|6|7.2
• Sample Data: Concentration(nmol/L)|0|0.031|0.062|0.125|0.25|0.5|1|2; O.D.|0.021|0.090|0.153|0.287|0.543|0.973|1.613|2.209
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Standard Curve (Sample)

(Human SHBG PicoKine ELISA Kit standard curve)

Related Product Information for SHBG elisa kit

Principle of the assay: Human SHBG ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SHBG has been precoated onto 96-well plates. Standards(NSO, L30-H402) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SHBG is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human SHBG amount of sample captured in plate.

Background: Sex hormone-binding globulin (SHBG) or sex steroid-binding globulin (SSBG) is a glycoprotein that binds to the two sex hormones: androgen and estrogen. The gene for SHBG is located on chromosome 17 on the short arm between the bands 17p12†'p13. And this gene encodes a steroid binding protein that was first described as a plasma protein secreted by the liver but is now thought to participate in the regulation of steroid responses. The encoded protein transports androgens and estrogens in the blood, binding each steroid molecule as a dimer formed from identical or nearly identical monomers. Polymorphisms in this gene have been associated with polycystic ovary syndrome and type 2 diabetes mellitus. Alternative splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

• NCBI GI #: 7382460
• NCBI GeneID: 6462
• NCBI Accession #: NP_001031.2
• NCBI GenBank Nucleotide #: NM_001040.4
• UniProt Accession #: P04278; P14689; Q16616; Q3MIL0; Q6ISD2; B0FWH4; E9PGW1; F5H5Z8; I3L1N7
• Molecular Weight: 41,712 Da
• NCBI Official Full Name: sex hormone-binding globulin isoform 1
• NCBI Official Synonym Full Names: sex hormone binding globulin
• NCBI Official Symbol: SHBG
• NCBI Official Synonym Symbols: ABP; SBP; TEBG
• NCBI Protein Information: sex hormone-binding globulin
• UniProt Protein Name: Sex hormone-binding globulin
• UniProt Gene Name: SHBG
• UniProt Synonym Gene Names: SHBG; SBP; ABP; TeBG
• UniProt Entry Name: SHBG_HUMAN