Human Siglec5 ELISA Kit | SIGLEC5 elisa kit

Human Siglec5 PicoKine ELISA Kit

Cat. Num. AAA46804

• Gene Names: SIGLEC5; CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
• Reactivity: Human
• Synonyms: Siglec5; N/A; Human Siglec5 PicoKine ELISA Kit; CD170; CD170 antigen; CD33L2; OB-BP2; OBBP2; Siglec-5; Siglecf; O15389; Sialic acid-binding Ig-like lectin 5; SIGLEC5 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human Siglec5
• Sequence Length: 551
• Samples: Cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA)
• Assay Type: Quantitative Sandwich
• Detection Range: 46.9pg/ml-3000pg/ml
• Sensitivity: <15pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Standard Curve (Sample)

(Human Siglec5 PicoKine ELISA Kit standard curve)

Related Product Information for SIGLEC5 elisa kit

Principle of the Assay: The Human Siglec5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Siglec5 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: E17-L441) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Siglec5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Siglec5 amount of sample captured in plate.

Background: Sialic acid-binding Ig-like lectin 5 is a protein that in humans is encoded by the SIGLEC5 gene. This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion.

NCBI and Uniprot Product Information

• NCBI GI #: 4502659
• NCBI GeneID: 8778
• NCBI Accession #: NP_003821.1
• NCBI GenBank Nucleotide #: NM_003830.3
• UniProt Accession #: O15389
• Molecular Weight: 60,715 Da
• NCBI Official Full Name: sialic acid-binding Ig-like lectin 5
• NCBI Official Synonym Full Names: sialic acid binding Ig like lectin 5
• NCBI Official Symbol: SIGLEC5
• NCBI Official Synonym Symbols: CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
• NCBI Protein Information: sialic acid-binding Ig-like lectin 5
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 5
• UniProt Gene Name: SIGLEC5
• UniProt Synonym Gene Names: CD33L2; OBBP2; Siglec-5; OB-BP2; OB-binding protein 2