Rabbit anti-Human, mouse SIGLEC5 Polyclonal Antibody | anti-SIGLEC5 antibody

SIGLEC5 Antibody (C-term)

Cat. Num. AAA288052

• Gene Names: SIGLEC5; CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
• Reactivity: Human, mouse
• Applications: ELISA, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: SIGLEC5, Antibody; SIGLEC5 Antibody (C-term); Sialic acid-binding Ig-like lectin 5; Siglec-5; CD33 antigen-like 2; Obesity-binding protein 2; OB-BP2; OB-binding protein 2; CD170; SIGLEC5; CD33L2; OBBP2; anti-SIGLEC5 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This SIGLEC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 482-513 amino acids from the C-terminal region of human SIGLEC5.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 482-513
• Sequence Length: 551

• Applicable Applications for anti-SIGLEC5 antibody: ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(SIGLEC5 Antibody western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the SIGLEC5 antibody detected the SIGLEC5 protein (arrow).)

WB (Western Blot)

(The anti-Siglec5 C-term Pab is used in Western blot to detect Siglec5 in mouse liver tissue lysate.)

Related Product Information for anti-SIGLEC5 antibody

SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have one or more cytoplasmic immune receptor tyrosine-based inhibitory motifs (ITIM). SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6. Sequence analysis predicted that the 697-amino acid SIGLEC10 protein contains a signal peptide, an N-terminal V-set Ig-like domain and four C2-set Ig-like domains, five potential N-linked glycosylation sites, a transmembrane region, and a 126-residue cytoplasmic tail with 3 putative ITIMs. Northern blot analysis detected a major 3.0-kb SIGLEC10 transcript, with highest levels in spleen, lymph node, blood leukocytes, and appendix. Little or no expression was observed in pancreas, thyroid, and testis. Flow cytometric analysis demonstrated eosinophil-specific expression of SIGLEC10, but at a lower level than that of SIGLEC8. Expression was also detected on monocytes and a CD16-positive/CD56-negative natural killer-like lymphocyte population. After sialidase treatment, which is necessary for unmasking the sialic acid-binding site on SIGLECs interacting with cell surface sialic acids, cells expressing SIGLEC10 bound to red blood cells. Immunoprecipitation analysis indicated expression of a 100- to 120-kD monomeric protein, higher than the predicted molecular mass, suggesting that SIGLEC10 is glycosylated.

Product Categories/Family for anti-SIGLEC5 antibody

Immunology

References

Erickson-Miller, C.L., et al., Exp. Hematol. 31(5):382-388 (2003).
Patel, N., et al., J. Biol. Chem. 274(32):22729-22738 (1999).
Cornish, A.L., et al., Blood 92(6):2123-2132 (1998).
Kim, H.S., Cytogenet. Cell Genet. 84 (1-2), 96 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 4502659
• NCBI GeneID: 8778
• NCBI Accession #: NP_003821.1
• NCBI GenBank Nucleotide #: NM_003830.3
• UniProt Accession #: O15389
• Molecular Weight: 60715
• NCBI Official Full Name: sialic acid-binding Ig-like lectin 5
• NCBI Official Synonym Full Names: sialic acid binding Ig-like lectin 5
• NCBI Official Symbol: SIGLEC5
• NCBI Official Synonym Symbols: CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
• NCBI Protein Information: sialic acid-binding Ig-like lectin 5
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 5
• UniProt Gene Name: SIGLEC5
• UniProt Synonym Gene Names: CD33L2; OBBP2; Siglec-5; OB-BP2; OB-binding protein 2
• UniProt Entry Name: SIGL5_HUMAN