Standard Curve (Sample)
(Fig.1. Human Ski-like protein (SKIL) Standard Curve.)
Human Ski-Like Protein (SKIL) ELISA Kit | SKIL elisa kit
Human Ski-Like Protein (SKIL) ELISA Kit
Gene Names
SKIL; SNO; SnoA; SnoI; SnoN
Reactivity
Human
Synonyms
Ski-Like Protein (SKIL); N/A; Human Ski-Like Protein (SKIL) ELISA Kit; SKIL; SNO; SnoA; SnoI; SnoN; SKI-like; ski-related oncogene snoN; SKIL elisa kit
Reactivity
Human
Specificity
Human Ski-like protein (SKIL) ELISA Kit has high sensitivity and excellent specificity for detection of Human SKIL. No significant cross-reactivity or interference between Human SKIL and analogues was observed.
Sequence Length
638
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Ski-like protein (SKIL) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Ski-like protein (SKIL) Standard Curve.)
Related Product Information for SKIL elisa kit
Description: This Human Ski-like protein (SKIL) ELISA Kit employs a two-site sandwich ELISA to quantitate SKIL in samples. An antibody specific for SKIL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SKIL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SKIL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SKIL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The mouse Sno gene expresses 2 isoforms, SnoN and SnoN2, that are different from each other in a location downstream of the site of alternative splicing previously described in the human SNO gene. SnoN2 is missing a 138-bp coding segment present in mouse SnoN and human SNON. TGFB induces activation and nuclear translocation of SMAD2, SMAD3, and SMAD4. SMAD3 causes degradation of SnoN, allowing a SMAD2/SMAD4 complex to activate TGFB target genes. To initiate a negative feedback mechanism that permits a precise and timely regulation of TGFB signaling, TGFB also induces an increased expression of SnoN at a later stage, which in turn binds to SMAD heteromeric complexes and shuts off TGFB signaling.
Background: The mouse Sno gene expresses 2 isoforms, SnoN and SnoN2, that are different from each other in a location downstream of the site of alternative splicing previously described in the human SNO gene. SnoN2 is missing a 138-bp coding segment present in mouse SnoN and human SNON. TGFB induces activation and nuclear translocation of SMAD2, SMAD3, and SMAD4. SMAD3 causes degradation of SnoN, allowing a SMAD2/SMAD4 complex to activate TGFB target genes. To initiate a negative feedback mechanism that permits a precise and timely regulation of TGFB signaling, TGFB also induces an increased expression of SnoN at a later stage, which in turn binds to SMAD heteromeric complexes and shuts off TGFB signaling.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ski-like protein isoform 2
NCBI Official Synonym Full Names
SKI like proto-oncogene
NCBI Official Symbol
SKIL
NCBI Official Synonym Symbols
SNO; SnoA; SnoI; SnoN
NCBI Protein Information
ski-like protein
UniProt Protein Name
Ski-like protein
UniProt Gene Name
SKIL
UniProt Synonym Gene Names
SNO
UniProt Entry Name
SKIL_HUMAN
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Product Notes
The Human SKIL skil (Catalog #AAA330858) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330858 ELISA Kit recognizes Human SKIL. It is sometimes possible for the material contained within the vial of "Ski-Like Protein (SKIL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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