Human Small Breast Epithelial Mucin (SBEM) ELISA Kit | SBEM elisa kit

Human Small Breast Epithelial Mucin (SBEM) ELISA Kit

Cat. Num. AAA185143

• Gene Names: MUCL1; SBEM
• Reactivity: Human
• Synonyms: Small Breast Epithelial Mucin (SBEM); N/A; Human Small Breast Epithelial Mucin (SBEM) ELISA Kit; MUCL1; Mucin-Like Protein 1; Protein BS106; Mucin-like protein 1; SBEM elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Small Breast Epithelial Mucin (SBEM).; No significant cross-reactivity or interference between Small Breast Epithelial Mucin (SBEM) and analogues was observed.
• Sequence Length: 90
• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Saliva and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.129ng/mL

• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Small Breast Epithelial Mucin (SBEM) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Small Breast Epithelial Mucin (SBEM) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for SBEM elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Small Breast Epithelial Mucin (SBEM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Small Breast Epithelial Mucin (SBEM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Small Breast Epithelial Mucin (SBEM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Small Breast Epithelial Mucin (SBEM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 15559111
• NCBI GeneID: 118430
• Molecular Weight: 9,039 Da
• NCBI Official Full Name: small breast epithelial mucin
• NCBI Official Synonym Full Names: mucin like 1
• NCBI Official Symbol: MUCL1
• NCBI Official Synonym Symbols: SBEM
• NCBI Protein Information: mucin-like protein 1
• UniProt Protein Name: Mucin-like protein 1
• UniProt Gene Name: MUCL1
• UniProt Synonym Gene Names: SBEM