WB (Western Blot)
( Western Blot using anti-breast epithelial mucin antibody Mc5 (Ab00345) MCF7 cell lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of Mc5 (Ab00345-23.0) at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified breast epithelial mucin is 122.1kDa, but this protein is heavily glycosylated and lipidated at several positions leading to the observed running size.)
Breast Epithelial Mucin Recombinant Antibody | BEM recombinant antibody
Anti-Breast Epithelial Mucin [Mc5]
Reactivity
Human
Applications
ELISA
Purity
Protein A Affinity Purified
Synonyms
Breast Epithelial Mucin, Recombinant Antibody; Anti-Breast Epithelial Mucin [Mc5]; BEM recombinant antibody
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG, Kappa
Clone Number
Mc5
Specificity
Specifically binds BEM.
Purity/Purification
Protein A Affinity Purified
Concentration
1 mg/ml (varies by lot)
Applicable Applications for BEM recombinant antibody
ELISA
Immunogen
Human breast epithelial mucin.
Buffer Composition
PBS only with 0.02% Proclin 300.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2 format, for improved compatibility with existing reagents, assays and techniques.
Labeled Substance
None
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
WB (Western Blot)
( Western Blot using anti-breast epithelial mucin antibody Mc5 (Ab00345) MCF7 cell lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of Mc5 (Ab00345-23.0) at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified breast epithelial mucin is 122.1kDa, but this protein is heavily glycosylated and lipidated at several positions leading to the observed running size.)
WB (Western Blot)
( Western Blot using anti-breast epithelial mucin antibody Mc5 (Ab00345) MCF7 cell lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of Mc5 (Ab00345-23.0) at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified breast epithelial mucin is 122.1kDa, but this protein is heavily glycosylated and lipidated at several positions leading to the observed running size.)
IF (Immunofluorescence)
( Immunofluorescence staining of fixed MCF7 cells with anti-Breast epithelial mucin antibody Mc5 (Ab00345) Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of Mc5 (Ab00345-23.0) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00345-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody (Ab178-23.0) followed by Alexa Fluor® 488 secondary antibody.)
IF (Immunofluorescence)
( Immunofluorescence staining of fixed MCF7 cells with anti-Breast epithelial mucin antibody Mc5 (Ab00345) Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of Mc5 (Ab00345-23.0) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00345-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody (Ab178-23.0) followed by Alexa Fluor® 488 secondary antibody.)
Related Product Information for BEM recombinant antibody
Recombinant monoclonal antibody to Breast epithelial mucin. Manufactured using a proprietary Recombinant Platform with variable regions (i.e. specificity) from the hybridoma Mc5.
Human breast epithelial mucin.
Human breast epithelial mucin.
Product Categories/Family for BEM recombinant antibody
References
Christian et al. 1996. Cloning and expression of cDNAs encoding the variable domains of the anti-breast carcinoma antibody Mc5. Hybridoma 1996; 15(2):155-158 PMID:8743296
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Product Notes
The BEM (Catalog #AAA71988) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Breast Epithelial Mucin [Mc5] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Breast Epithelial Mucin can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the BEM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Breast Epithelial Mucin, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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