Standard Curve (Sample)
Human Small Proline-Rich Protein 2A (SPRR2A) ELISA Kit | SPRR2A elisa kit
Human Small Proline-Rich Protein 2A (SPRR2A) ELISA Kit
Reactivity
Human
Synonyms
Small Proline-Rich Protein 2A (SPRR2A); N/A; Human Small Proline-Rich Protein 2A (SPRR2A) ELISA Kit; SPRR2A elisa kit
Reactivity
Human
Specificity
Human Small proline-rich protein 2A (SPRR2A) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPRR2A. No significant cross-reactivity or interference between Human SPRR2A and analogues was observed.
Sequence Length
72
Assay Type
Sandwich
Samples
Human serum, cell culture supernates and other biological fluids
Detection Range
30 pg/ml - 480 pg/ml
Sensitivity
< 1pg/ml
Intra-assay Precision
Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?9%.
Inter-assay Precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?11%.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SPRR2A elisa kit
Background: SPRR genes encode a novel class of polypeptides called small proline-rich proteins that are strongly induced during differentiation of human epidermal keratinocytes in vitro and in vivo. Small proline-rich protein 2A is a protein encoded by the SPRR2A gene.Gibbs et al. (1993) concluded that there are 7 genes for the SPRR2 subfamily of small proline-rich proteins, including SPRR2A.Using DNA microarray analysis, Zimmermann et al. (2005) identified Sprr2a and Sprr2b (182268) as allergen-induced genes in a mouse model of experimental asthma.
Principle of the Assay: Human Small proline-rich protein 2A (SPRR2A) ELISA Kit employs a two-site sandwich ELISA to quantitate SPRR2A in samples. An antibody specific for SPRR2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPRR2A present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human SPRR2A detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of SPRR2A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: Human Small proline-rich protein 2A (SPRR2A) ELISA Kit employs a two-site sandwich ELISA to quantitate SPRR2A in samples. An antibody specific for SPRR2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPRR2A present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human SPRR2A detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of SPRR2A bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
small proline-rich protein 2A
NCBI Official Synonym Full Names
small proline rich protein 2A
NCBI Official Symbol
SPRR2A
NCBI Protein Information
small proline-rich protein 2A
UniProt Protein Name
Small proline-rich protein 2A
UniProt Gene Name
SPRR2A
UniProt Synonym Gene Names
SPR-2A
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Product Notes
The Human SPRR2A sprr2a (Catalog #AAA330565) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330565 ELISA Kit recognizes Human SPRR2A. It is sometimes possible for the material contained within the vial of "Small Proline-Rich Protein 2A (SPRR2A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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