Standard Curve (Sample)
(Fig.1. Human Small proline-rich protein 2B (SPRR2B) Standard Curve.)
Human Small Proline-Rich Protein 2B (SPRR2B) ELISA Kit | SPRR2B elisa kit
Human Small Proline-Rich Protein 2B (SPRR2B) ELISA Kit
Reactivity
Human
Synonyms
Small Proline-Rich Protein 2B (SPRR2B); N/A; Human Small Proline-Rich Protein 2B (SPRR2B) ELISA Kit; SPRR2B; OTTHUMP00000015752; SPRR2B elisa kit
Reactivity
Human
Specificity
Human Small proline-rich protein 2B (SPRR2B) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPRR2B. No significant cross-reactivity or interference between Human SPRR2B and analogues was observed.
Sequence Length
72
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Small proline-rich protein 2B (SPRR2B) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Small proline-rich protein 2B (SPRR2B) Standard Curve.)
Related Product Information for SPRR2B elisa kit
Description: This Human Small proline-rich protein 2B (SPRR2B) ELISA Kit employs a two-site sandwich ELISA to quantitate SPRR2B in samples. An antibody specific for SPRR2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPRR2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPRR2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPRR2B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SPRR genes encode a novel class of polypeptides called small proline-rich proteins that are strongly induced during differentiation of human epidermal keratinocytes in vitro and in vivo. SPRR genes constitute a distinct class of cornified envelope precursor proteins. Gibbs et al. (1993) concluded that there are 7 genes for the SPRR2 subfamily of small proline-rich proteins, including SPRR2B.Using DNA microarray analysis, Zimmermann et al. (2005) identified Sprr2a and Sprr2b as allergen-induced genes in a mouse model of experimental asthma.By analysis of human-rodent hybrid cell lines and FISH, Gibbs et al. (1993) determined that all SPRR genes, including SPRR2B, are closely linked within a 300-kb DNA segment on chromosome 1q21-q22.
Background: SPRR genes encode a novel class of polypeptides called small proline-rich proteins that are strongly induced during differentiation of human epidermal keratinocytes in vitro and in vivo. SPRR genes constitute a distinct class of cornified envelope precursor proteins. Gibbs et al. (1993) concluded that there are 7 genes for the SPRR2 subfamily of small proline-rich proteins, including SPRR2B.Using DNA microarray analysis, Zimmermann et al. (2005) identified Sprr2a and Sprr2b as allergen-induced genes in a mouse model of experimental asthma.By analysis of human-rodent hybrid cell lines and FISH, Gibbs et al. (1993) determined that all SPRR genes, including SPRR2B, are closely linked within a 300-kb DNA segment on chromosome 1q21-q22.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
small proline-rich protein 2B
NCBI Official Synonym Full Names
small proline rich protein 2B
NCBI Official Symbol
SPRR2B
NCBI Protein Information
small proline-rich protein 2B
UniProt Protein Name
Small proline-rich protein 2B
UniProt Gene Name
SPRR2B
UniProt Synonym Gene Names
SPR-2B
UniProt Entry Name
SPR2B_HUMAN
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Product Notes
The Human SPRR2B sprr2b (Catalog #AAA330566) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330566 ELISA Kit recognizes Human SPRR2B. It is sometimes possible for the material contained within the vial of "Small Proline-Rich Protein 2B (SPRR2B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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