Standard Curve (Sample)
(Fig.1. Human Sodium/bile acid cotransporter (SLC10A1) Standard Curve.)
Human Sodium/Bile Acid Cotransporter (SLC10A1) ELISA Kit | SLC10A1 elisa kit
Human Sodium/Bile Acid Cotransporter (SLC10A1) ELISA Kit
Gene Names
SLC10A1; NTCP
Reactivity
Human
Synonyms
Sodium/Bile Acid Cotransporter (SLC10A1); N/A; Human Sodium/Bile Acid Cotransporter (SLC10A1) ELISA Kit; SLC10A1; NTCP; Na/taurocholate cotransporting polypeptide; growth-inhibiting protein 29; sodium/bile acid cotransporter; sodium/taurocholate cotransporter; solute carrier family 10; member 1; SLC10A1 elisa kit
Reactivity
Human
Specificity
Human Sodium/bile acid cotransporter (SLC10A1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC10A1. No significant cross-reactivity or interference between Human SLC10A1 and analogues was observed.
Sequence Length
349
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Sodium/bile acid cotransporter (SLC10A1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Sodium/bile acid cotransporter (SLC10A1) Standard Curve.)
Related Product Information for SLC10A1 elisa kit
Description: This Human Sodium/bile acid cotransporter (SLC10A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC10A1 in samples. An antibody specific for SLC10A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC10A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC10A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC10A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Sodium/bile acid cotransporters are integral membrane glycoproteins. Two homologous transporters are involved in the reabsorption of bile acids, one absorbing from the intestinal lumen, the bile duct, and the kidney with an apical localization (SLC10A2;), and the other being found in the basolateral membranes of hepatocytes (SLC10A1). SLC10A1 contains an exoplasmic N terminus, an odd number of transmembrane regions, and a cytoplasmic C terminus. Alanine insertion experiments confirmed that 7 of the 9 hydrophobic stretches are membrane-integrated, with secondary structures and transport activity sensitive to positional displacement. Two amphipathic sequences are critical for intramolecular interactions and for proper trafficking of SLC10A1 to the plasma membrane.
Background: Sodium/bile acid cotransporters are integral membrane glycoproteins. Two homologous transporters are involved in the reabsorption of bile acids, one absorbing from the intestinal lumen, the bile duct, and the kidney with an apical localization (SLC10A2;), and the other being found in the basolateral membranes of hepatocytes (SLC10A1). SLC10A1 contains an exoplasmic N terminus, an odd number of transmembrane regions, and a cytoplasmic C terminus. Alanine insertion experiments confirmed that 7 of the 9 hydrophobic stretches are membrane-integrated, with secondary structures and transport activity sensitive to positional displacement. Two amphipathic sequences are critical for intramolecular interactions and for proper trafficking of SLC10A1 to the plasma membrane.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Sodium/bile acid cotransporter
NCBI Official Synonym Full Names
solute carrier family 10 member 1
NCBI Official Symbol
SLC10A1
NCBI Official Synonym Symbols
NTCP
NCBI Protein Information
sodium/bile acid cotransporter
UniProt Protein Name
Sodium/bile acid cotransporter
UniProt Gene Name
SLC10A1
UniProt Synonym Gene Names
NTCP
UniProt Entry Name
NTCP_HUMAN
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Product Notes
The Human SLC10A1 slc10a1 (Catalog #AAA330854) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330854 ELISA Kit recognizes Human SLC10A1. It is sometimes possible for the material contained within the vial of "Sodium/Bile Acid Cotransporter (SLC10A1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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