Standard Curve (Sample)
(Fig.1. Human Soluble OX40L (sOX40L) Standard Curve.)
Human Soluble OX40L (SOX40L) ELISA Kit | SOX40L elisa kit
Human Soluble OX40L (SOX40L) ELISA Kit
Gene Names
TNFSF4; GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; TNLG2B
Reactivity
Human
Synonyms
Soluble OX40L (SOX40L); N/A; Human Soluble OX40L (SOX40L) ELISA Kit; TNFSF4; CD134L; CD252; GP34; OX-40L; OX4OL; TXGP1; CD134 ligand; OX40 antigen ligand; tax-transcriptionally activated glycoprotein 1 (34kD); SOX40L elisa kit
Reactivity
Human
Specificity
Human Soluble OX40L (sOX40L) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNFSF4. No significant cross-reactivity or interference between Human TNFSF4 and analogues was observed.
Sequence Length
183
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Soluble OX40L (sOX40L) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Soluble OX40L (sOX40L) Standard Curve.)
Related Product Information for SOX40L elisa kit
Description: This Human Soluble OX40L (sOX40L) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF4 in samples. An antibody specific for TNFSF4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNFSF4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFSF4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFSF4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: sOX40L encodes a cytokine of the tumor necrosis factor (TNF) ligand family. Soluble OX40L functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants.
Background: sOX40L encodes a cytokine of the tumor necrosis factor (TNF) ligand family. Soluble OX40L functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 4 isoform 1
NCBI Official Synonym Full Names
TNF superfamily member 4
NCBI Official Symbol
TNFSF4
NCBI Official Synonym Symbols
GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; TNLG2B
NCBI Protein Information
tumor necrosis factor ligand superfamily member 4
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 4
UniProt Gene Name
TNFSF4
UniProt Synonym Gene Names
TXGP1; OX40L
UniProt Entry Name
TNFL4_HUMAN
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Product Notes
The Human SOX40L tnfsf4 (Catalog #AAA330422) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330422 ELISA Kit recognizes Human SOX40L. It is sometimes possible for the material contained within the vial of "Soluble OX40L (SOX40L), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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