Rabbit anti-Human TNFSF4 / OX40L Polyclonal Antibody | anti-TNFSF4 / OX40L antibody

Anti-TNFSF4 / OX40L Antibody (aa102-131) IHC-plus

Cat. Num. AAA52668

• Gene Names: TNFSF4; GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; TNLG2B
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry
• Purity: Protein A purified
• Synonyms: TNFSF4 / OX40L, Antibody; Anti-TNFSF4 / OX40L Antibody (aa102-131) IHC-plus; Rabbit Polyclonal to Human TNFSF4 / OX40L; Human TNFSF4 / OX40L; CD134 ligand; CD134L; CD252 antigen; Glycoprotein Gp34; OX-40L; OX40 ligand; TXGP1; OX4OL; CD252; gp34; OX40 antigen ligand; anti-TNFSF4 / OX40L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: This TNFSF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-131 amino acids from the Central region of human TNFSF4.
• Purity/Purification: Protein A purified
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 183

• Applicable Applications for anti-TNFSF4 / OX40L antibody: WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry)
• Immunogen Species: TNFS4/OX40L antibody was raised against Human.
• Family/Subfamily: TNF/not assigned-TNF
• Epitope: aa102-131
• Target: TNFSF4/OX40L
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 6 months.; For long term storage store at -20°C.

WB (Western Blot)

(TNFSF4 Antibody western blot of CEM cell line lysates (35 ug/lane). The TNFSF4 antibody detected the TNFSF4 protein (arrow).)

IHC (Immunohiostchemistry)

(Anti-TNFSF4 / OX40L antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

IHC (Immunohistochemistry)

(Anti-TNFSF4 / OX40L antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-TNFSF4 / OX40L antibody

TNFSF4 Antibody, CD134 ligand Antibody, CD134L Antibody, CD252 antigen Antibody, Glycoprotein Gp34 Antibody, OX-40L Antibody, OX40 ligand Antibody, TXGP1 Antibody, OX40L Antibody, OX4OL Antibody, CD252 Antibody, gp34 Antibody, OX40 antigen ligand Antibody Description: TNFSF4 / OX40L is a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

• NCBI GI #: 4507603
• NCBI GeneID: 7292
• NCBI Accession #: NP_003317.1
• NCBI GenBank Nucleotide #: NM_003326.4
• UniProt Accession #: P23510; Q5JZA5; Q8IV74; Q9HCN9
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 4 isoform 1
• NCBI Official Synonym Full Names: tumor necrosis factor superfamily member 4
• NCBI Official Symbol: TNFSF4
• NCBI Official Synonym Symbols: GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; TNLG2B
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 4
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 4
• UniProt Gene Name: TNFSF4
• UniProt Synonym Gene Names: TXGP1; OX40L
• UniProt Entry Name: TNFL4_HUMAN