Standard Curve (Sample)
Human SPHK1 ELISA Kit | SPHK1 elisa kit
Human SPHK1 ELISA Kit
Gene Names
SPHK1; SPHK
Reactivity
Human
Synonyms
SPHK1; N/A; Human SPHK1 ELISA Kit; SPHK; SPK; Sphingosine kinase 1; SK 1; SPK 1; SPHK1 elisa kit
Reactivity
Human
Specificity
The Human SPHK1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human SPHK1 proteins within the range of 312 pg/ml - 20000 pg/ml.
Sequence Length
384
Samples
Culture Supernates, Serum, Plasma
Sensitivity
< 10 pg/ml
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SPHK1 elisa kit
Background: Sphingosine kinase 1 is an enzyme that in humans is encoded by the SPHK1 gene. The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1- phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Principle of the Assay: The Human SPHK1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SPHK1 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human SPHK1 coated on a 96-well plate. Standards and samples are pipetted into the wells and SPHK1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SPHK1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SPHK1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Principle of the Assay: The Human SPHK1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SPHK1 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human SPHK1 coated on a 96-well plate. Standards and samples are pipetted into the wells and SPHK1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SPHK1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SPHK1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,944 Da
NCBI Official Full Name
sphingosine kinase 1 isoform 3
NCBI Official Synonym Full Names
sphingosine kinase 1
NCBI Official Symbol
SPHK1
NCBI Official Synonym Symbols
SPHK
NCBI Protein Information
sphingosine kinase 1
UniProt Protein Name
Sphingosine kinase 1
UniProt Gene Name
SPHK1
UniProt Synonym Gene Names
SPHK; SPK; SK 1; SPK 1
UniProt Entry Name
SPHK1_HUMAN
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Product Notes
The Human SPHK1 sphk1 (Catalog #AAA263086) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA263086 ELISA Kit recognizes Human SPHK1. It is sometimes possible for the material contained within the vial of "SPHK1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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