Standard Curve (Sample)
Human SPINK5 ELISA Kit | SPINK5 elisa kit
Human SPINK5 PicoKine ELISA Kit
Gene Names
SPINK5; NS; NETS; LEKTI; LETKI; VAKTI
Reactivity
Human
Synonyms
SPINK5; N/A; Human SPINK5 PicoKine ELISA Kit; Human SPINK5; serine peptidase inhibitor, Kazal type 5; Serine protease inhibitor Kazal-type 5; Lympho-epithelial Kazal-type-related inhibitor; LEKTI; SPINK5 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human SPINK5
Sequence Length
1094
Samples
Cell culture supernatants, serum and plasma (heparin, edta, citrate)
Detection Range
312 pg/ml - 20,000 pg/ml
Sensitivity
<50 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SPINK5 elisa kit
Principle of the Assay: The Picokine™ Human SPINK5 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human SPINK5 with a 96-well strip plate that is pre-coated with antibody specific for SPINK5. The detection antibody is a biotinylated antibody specific for SPINK5. The capture antibody is a monoclonal antibody from mouse and the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human SPINK5 with immunogen: Expression system for standard: NS0; Immunogen sequence: K23-E1064. The kit is analytically validated with ready-to-use reagents. To measure Human SPINK5, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human SPINK5 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human SPINK5 in the sample.
Background/Introduction: Lympho-epithelial Kazal-type-related inhibitor (LEKTI) also known as serine protease inhibitor Kazal-type 5 (SPINK5) is a protein that in humans is encoded by the SPINK5 gene. This gene is mapped to 5q32. This gene encodes a multidomain serine protease inhibitor that contains 15 potential inhibitory domains. The encoded preproprotein is proteolytically processed to generate multiple protein products, which may exhibit unique activities and specificities. These proteins may play a role in skin and hair morphogenesis, as well as anti-inflammatory and antimicrobial protection of mucous epithelia. Mutations in this gene may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy. This gene is present in a gene cluster on chromosome 5. Alternative splicing results in multiple transcript variants.
Background/Introduction: Lympho-epithelial Kazal-type-related inhibitor (LEKTI) also known as serine protease inhibitor Kazal-type 5 (SPINK5) is a protein that in humans is encoded by the SPINK5 gene. This gene is mapped to 5q32. This gene encodes a multidomain serine protease inhibitor that contains 15 potential inhibitory domains. The encoded preproprotein is proteolytically processed to generate multiple protein products, which may exhibit unique activities and specificities. These proteins may play a role in skin and hair morphogenesis, as well as anti-inflammatory and antimicrobial protection of mucous epithelia. Mutations in this gene may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy. This gene is present in a gene cluster on chromosome 5. Alternative splicing results in multiple transcript variants.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
serine protease inhibitor Kazal-type 5 isoform a preproprotein
NCBI Official Synonym Full Names
serine peptidase inhibitor, Kazal type 5
NCBI Official Symbol
SPINK5
NCBI Official Synonym Symbols
NS; NETS; LEKTI; LETKI; VAKTI
NCBI Protein Information
serine protease inhibitor Kazal-type 5
UniProt Protein Name
Serine protease inhibitor Kazal-type 5
UniProt Gene Name
SPINK5
UniProt Synonym Gene Names
LEKTI
UniProt Entry Name
ISK5_HUMAN
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Product Notes
The Human SPINK5 spink5 (Catalog #AAA125250) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA125250 ELISA Kit recognizes Human SPINK5. It is sometimes possible for the material contained within the vial of "SPINK5, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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