IF (Immunofluorescence)
(AAA325112 staining NIH-3T3 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Rabbit anti-Human, Mouse SPINK5 Polyclonal Antibody | anti-SPINK5 antibody
SPINK5 Antibody
Gene Names
SPINK5; NS; NETS; LEKTI; LETKI; VAKTI
Reactivity
Human, Mouse
Purity
Peptide affinity purification
Synonyms
SPINK5, Antibody; SPINK5 Antibody; Hemofiltrate peptide HF7665; ISK5_HUMAN; LEKTI; Lympho-epithelial Kazal-type-related inhibitor; Lymphoepithelial Kazal type related inhibitor; NETS; NS; Serine peptidase inhibitor Kazal type 5; SPINK5; VAKTI; anti-SPINK5 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
SPINK5 Antibody detects endogenous levels of total SPINK5.
Purity/Purification
Peptide affinity purification
Form/Format
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Immunogen
A synthesized peptide derived from human SPINK5, corresponding to a region within N-terminal amino acids.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA325112 staining NIH-3T3 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA325112 staining NIH-3T3 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from NIH/3T3 cells using SPINK5 antibody.)
WB (Western Blot)
(Western blot analysis of extracts from NIH/3T3 cells using SPINK5 antibody.)
Related Product Information for anti-SPINK5 antibody
Serine protease inhibitor, probably important for the anti-inflammatory and/or antimicrobial protection of mucous epithelia. Contribute to the integrity and protective barrier function of the skin by regulating the activity of defense-activating and desquamation-involved proteases. Inhibits KLK5, it's major target, in a pH-dependent manner. Inhibits KLK7, KLK14 CASP14, and trypsin.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
121 KD
NCBI Official Full Name
serine protease inhibitor Kazal-type 5 isoform a preproprotein
NCBI Official Synonym Full Names
serine peptidase inhibitor, Kazal type 5
NCBI Official Symbol
SPINK5
NCBI Official Synonym Symbols
NS; NETS; LEKTI; LETKI; VAKTI
NCBI Protein Information
serine protease inhibitor Kazal-type 5
UniProt Protein Name
Serine protease inhibitor Kazal-type 5
UniProt Gene Name
SPINK5
UniProt Synonym Gene Names
LEKTI
Similar Products
Product Notes
The SPINK5 spink5 (Catalog #AAA325112) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPINK5 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "SPINK5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.